Intrinsic Bioremediation of Petroleum Hydrocarbons in a Gas Condensate-Contaminated Aquifer

• Published in: Environmental science & technology 1999-08, Vol.33 (15), p.2550-2560
• Main Authors: Gieg, Lisa M, Kolhatkar, Ravindra V, McInerney, Michael J, Tanner, Ralph S, Harris, Steve H, Sublette, Kerry L, Suflita, Joseph M
• Format: Article
• Language: English
• Subjects: 02 PETROLEUM; 020900 - Petroleum- Environmental Aspects; ALKYLATED AROMATICS; Applied sciences; AQUIFERS; AROMATICS; BENZENE; BIODEGRADATION; Biological and physicochemical properties of pollutants. Interaction in the soil; Bioremediation; CHEMICAL REACTIONS; Chemistry; Contamination; DECOMPOSITION; Earth sciences; Earth, ocean, space; ENERGY SOURCES; Engineering and environment geology. Geothermics; Exact sciences and technology; FOSSIL FUELS; FUELS; HYDROCARBONS; OIL SPILLS; ORGANIC COMPOUNDS; PETROLEUM; Pollution; Pollution, environment geology; REMEDIAL ACTION; Soil and sediments pollution
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es981349d

A study was designed to determine if the intrinsic bioremediation of gas condensate hydrocarbons represented an important fate process in a shallow aquifer underlying a natural gas production site. For over 4 yr, changes in the groundwater, sediment, and vadose zone chemistry in the contaminated portion of the aquifer were interpreted relative to a background zone. Changes included decreased dissolved oxygen and sulfate levels and increased alkalinity, Fe(II), and methane concentrations in the contaminated groundwater, suggesting that aerobic heterotrophic respiration depleted oxygen reserves leaving anaerobic conditions in the hydrocarbon-impacted subsurface. Dissolved hydrogen levels in the contaminated groundwater indicated that sulfate reduction and methanogenesis were predominant biological processes, corroborating the geochemical findings. Furthermore, 10−1000-fold higher numbers of sulfate reducers and methanogens were enumerated in the contaminated sediment relative to background. Putative metabolites were also detected in the contaminated groundwater, including methylbenzylsuccinic acid, a signature intermediate of anaerobic xylene decay. Laboratory incubations showed that benzene, toluene, ethylbenzene, and each of the xylene isomers were biodegraded under sulfate-reducing conditions as was toluene under methanogenic conditions. These results coupled with a decrease in hydrocarbon concentrations in the contaminated sediment confirm that intrinsic bioremediation contributes to the attenuation of hydrocarbons in this aquifer.