Infrared Imaging of Sunflower and Maize Root Anatomy

Synchrotron radiation infrared microspectroscopy (SR−IMS) permits the direct analysis of plant cell-wall architecture at the cellular level in situ, combining spatially localized information and chemical information from IR absorbances to produce a chemical map that can be linked to a particular mor...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2007-12, Vol.55 (26), p.10517-10530
Main Authors: Dokken, Kenneth M, Davis, Lawrence C
Format: Article
Language:English
Subjects:
09 BIOMASS FUELS
Analytical Methods
ANATOMY
ARCHITECTURE
Biological and medical sciences
CELL WALL
CELLULOSE
Cereal and baking product industries
corn
EPIDERMIS
Food industries
FTIR microspectroscopy
FUNCTIONALS
Fundamental and applied biological sciences. Psychology
Helianthus - anatomy & histology
Helianthus annuus
Hydroponics
Infrared imaging
infrared radiation
LIGNIN
MAIZE
MORPHOLOGY
national synchrotron light source
PARTICLE ACCELERATORS
plant anatomy
plant proteins
Plant Roots - anatomy & histology
plant tissue
PLANT TISSUES
principal component analysis
principal components analysis
PROTEINS
roots
SPECTRA
Spectrophotometry, Infrared - methods
spectroscopy
SUNFLOWERS
SYNCHROTRON RADIATION
synchrotron radiation infrared microspectroscopy
Xylem - anatomy & histology
Zea mays
Zea mays - anatomy & histology
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Summary:Synchrotron radiation infrared microspectroscopy (SR−IMS) permits the direct analysis of plant cell-wall architecture at the cellular level in situ, combining spatially localized information and chemical information from IR absorbances to produce a chemical map that can be linked to a particular morphology or functional group. This study demonstrated the use of SR−IMS to probe biopolymers, such as cellulose, lignin, and proteins, in the root tissue of hydroponically grown sunflower and maize plants. Principal components analysis (PCA) was employed to reveal the major spectral variance between maize and sunflower plant tissues. The use of PCA showed distinct separation of maize and sunflower samples using the IR spectra of the epidermis and xylem. The infrared band at 1635 cm−1, representing hydrocinnamic acid in (H type) lignin, provided a conclusive means of distinguishing between maize and sunflower plant tissues.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf072052e