Chemometric analysis of multiple species of Bacillus bacterial endospores using infrared spectroscopy: Discrimination to the strain level

Previous work using infrared spectroscopy has shown potential for rapid discrimination between bacteria in either their sporulated or vegetative states, as well as between bacteria and other common interferents. For species within one physiological state, however, distinction is far more challenging...

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Bibliographic Details
Published in:Analytica chimica acta 2009-09, Vol.651 (1), p.24-30
Main Authors: Forrester, Joel B., Valentine, Nancy B., Su, Yin-Fong, Johnson, Timothy J.
Format: Article
Language:English
Subjects:
Analytical chemistry
BACILLUS
Bacillus - chemistry
Bacillus - classification
BACTERIA
Bacterial Typing Techniques - methods
BASIC BIOLOGICAL SCIENCES
Chemistry
Chemometrics
CLASSIFICATION
Discrimination
Endospores
Exact sciences and technology
FTIR
GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
GLUCOSE
Infrared spectroscopy
Principal Component Analysis
SPECTRA
Spectrometric and optical methods
Spectrophotometry, Infrared - methods
SPECTROSCOPY
SPORES
Spores, Bacterial - chemistry
Spores, Bacterial - classification
Spores, Bacterial - genetics
STRAINS
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Summary:Previous work using infrared spectroscopy has shown potential for rapid discrimination between bacteria in either their sporulated or vegetative states, as well as between bacteria and other common interferents. For species within one physiological state, however, distinction is far more challenging, and requires chemometrics. In the current study, we have narrowed the field of study by eliminating the confounding issues of vegetative cells as well as growth media and focused on using IR spectra to distinguish only between different species all in the sporulated state. Using principal component analysis (PCA) and a classification method based upon similarity measurements, we demonstrate a successful identification rate to the species level of 85% for Bacillus spores grown and sporulated in a glucose broth medium.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2009.08.005