Effects of hyperoxia on skeletal muscle carbohydrate metabolism during transient and steady-state exercise

• Published in: Journal of applied physiology (1985) 2005-01, Vol.98 (1), p.250-256
• Main Authors: Stellingwerff, Trent, Glazier, Lee, Watt, Matthew J, LeBlanc, Paul J, Heigenhauser, George J. F, Spriet, Lawrence L
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Carbohydrate Metabolism; Carbohydrates; Exercise; Exercise Test; Fundamental and applied biological sciences. Psychology; Humans; Hyperoxia - physiopathology; Lactic Acid - metabolism; Male; Metabolism; Muscle, Skeletal - physiopathology; Muscular system; Oxygen - metabolism; Oxygen Consumption; Physical Exertion; Pyruvate Dehydrogenase Complex - blood; Single-Blind Method; Striated muscle. Tendons; Time Factors; Vertebrates: osteoarticular system, musculoskeletal system
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.00897.2004

1 Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, and 2 Department of Medicine, McMaster University, Hamilton, Ontario, Canada Submitted 18 August 2004 ; accepted in final form 14 September 2004 This study compared the effects of inspiring either a hyperoxic (60% O 2 ) or normoxic gas (21% O 2 ) while cycling at 70% peak O 2 uptake on 1 ) the ATP derived from substrate phosphorylation during the initial minute of exercise, as estimated from phosphocreatine degradation and lactate accumulation, and 2 ) the reliance on carbohydrate utilization and oxidation during steady-state cycling, as estimated from net muscle glycogen use and the activity of pyruvate dehydrogenase (PDH) in the active form (PDH a ), respectively. We hypothesized that 60% O 2 would decrease substrate phosphorylation at the onset of exercise and that it would not affect steady-state exercise PDH activity, and therefore muscle carbohydrate oxidation would be unaltered. Ten active male subjects cycled for 15 min on two occasions while inspiring 21% or 60% O 2 , balance N 2 . Blood was obtained throughout and skeletal muscle biopsies were sampled at rest and 1 and 15 min of exercise in each trial. The ATP derived from substrate-level phosphorylation during the initial minute of exercise was unaffected by hyperoxia (21%: 52.2 ± 11.1; 60%: 54.0 ± 9.5 mmol ATP/kg dry wt). Net glycogen breakdown during 15 min of cycling was reduced during the 60% O 2 trial vs. 21% O 2 (192.7 ± 25.3 vs. 138.6 ± 16.8 mmol glycosyl units/kg dry wt). Hyperoxia had no effect on PDH a , because it was similar to the 21% O 2 trial at rest and during exercise (21%: 2.20 ± 0.26; 60%: 2.25 ± 0.30 mmol·kg wet wt –1 ·min –1 ). Blood lactate was lower (6.4 ± 1.0 vs. 8.9 ± 1.0 mM) at 15 min of exercise and net muscle lactate accumulation was reduced from 1 to 15 min of exercise in the 60% O 2 trial compared with 21% (8.6 ± 5.1 vs. 27.3 ± 5.8 mmol/kg dry wt). We concluded that O 2 availability did not limit oxidative phosphorylation in the initial minute of the normoxic trial, because substrate phosphorylation was unaffected by hyperoxia. Muscle glycogenolysis was reduced by hyperoxia during steady-state exercise, but carbohydrate oxidation (PDH a ) was unaffected. This closer match between pyruvate production and oxidation during hyperoxia resulted in decreased muscle and blood lactate accumulation. The mechanism responsible for the decreased muscle glycogenolysis during hyperoxia i