Microbubble sizing and shell characterization using flow cytometry

Experiments were performed to size, count, and obtain shell parameters for individual ultrasound contrast microbubbles using a modified flow cytometer. Light scattering was modeled using Mie theory, and applied to calibration beads to calibrate the system. The size distribution and population were m...

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Bibliographic Details
Published in:IEEE transactions on ultrasonics, ferroelectrics, and frequency control ferroelectrics, and frequency control, 2011-05, Vol.58 (5), p.955-963
Main Authors: Tu, J, Swalwell, J E, Giraud, D, Cui, W, Chen, W, Matula, T J
Format: Article
Language:English
Subjects:
Acoustics
Beads
Biological and medical sciences
Calibration
Cardiovascular system
Contrast agents
Contrast Media - chemistry
Elastic Modulus
Equipment Design
Flow Cytometry - instrumentation
Flow Cytometry - methods
Fluid flow measurement
Investigative techniques, diagnostic techniques (general aspects)
Mathematical model
Mathematical models
Medical sciences
Microbubbles
Microorganisms
Models, Chemical
Normal Distribution
Particle Size
Shear Strength
Shear viscosity
Shells
Size distribution
Studies
Transducers
Ultrasonic imaging
Ultrasonic investigative techniques
Ultrasonic variables measurement
Ultrasonography - instrumentation
Viscosity
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Summary:Experiments were performed to size, count, and obtain shell parameters for individual ultrasound contrast microbubbles using a modified flow cytometer. Light scattering was modeled using Mie theory, and applied to calibration beads to calibrate the system. The size distribution and population were measured directly from the flow cytometer. The shell parameters (shear modulus and shear viscosity) were quantified at different acoustic pressures (from 95 to 333 kPa) by fitting microbubble response data to a bubble dynamics model. The size distribution of the contrast agent microbubbles is consistent with manufacturer specifications. The shell shear viscosity increases with increasing equilibrium microbubble size, and decreases with increasing shear rate. The observed trends are independent of driving pressure amplitude. The shell elasticity does not vary with microbubble size. The results suggest that a modified flow cytometer can be an effective tool to characterize the physical properties of microbubbles, including size distribution, population, and shell parameters.
ISSN:0885-3010
1525-8955
DOI:10.1109/TUFFC.2011.1896