Differential Diagnosis Based on Immunological Phenotyping in Suspected Malignant Bone Marrow Involvement in Childhood

The diagnostic value of immunophenotyping (IP) as a first-line diagnostic method in diseases that infiltrate the childhood bone marrow (BM) or mimic infiltrated BM was examined. Two hundred and fifty unselected BM samples from 250 children suspected to have a malignancy infiltrating their BM were ev...

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Bibliographic Details
Published in:Pediatric hematology and oncology 1997, Vol.14 (1), p.29-41
Main Authors: Gussetis, Evgenios S., Schwabe, Dirk, Gerein, Valentin, Kornhuber, Bernhard
Format: Article
Language:English
Subjects:
Adolescent
Biological and medical sciences
bone marrow
Bone Marrow - pathology
Bone Marrow Neoplasms - diagnosis
Bone Marrow Neoplasms - immunology
Child
Child, Preschool
Diagnosis, Differential
differential diagnosis
Diseases of the osteoarticular system
Female
Hematologic Neoplasms - pathology
Host-tumor relations. Immunology. Biological markers
Humans
Immunophenotyping
Infant
Infant, Newborn
Leukemic Infiltration - diagnosis
Leukemic Infiltration - immunology
Male
malignant infiltration
Medical sciences
Prospective Studies
Sensitivity and Specificity
Tumors
Tumors of striated muscle and skeleton
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Summary:The diagnostic value of immunophenotyping (IP) as a first-line diagnostic method in diseases that infiltrate the childhood bone marrow (BM) or mimic infiltrated BM was examined. Two hundred and fifty unselected BM samples from 250 children suspected to have a malignancy infiltrating their BM were evaluated by means of IP and conventional morophological-cytochemical (MC) studies. We applied the alkaline phosphatase anti-alkaline phosphatase method for IP using a panel of monoclonal antibodies (Mabs) against leukocyte-associated antigens, neuroectodermal antigens, and intermediate filament antigens. Four cases of neuroblastoma, two cases of Ewing sarcoma, and one case of rhabdomyosarcoma were diagnosed by IP but not by MC studies. In nine cases of acute leukemia bone marrow blasts could not be ascribed to a specific lineage on the basis of blast morphology or histochemistry. Eight samples without morphological evidence of malignant infiltration revealed an increased percentage of immature B cell precursors (CD10+, TdT+) suggesting acute lymphoblastic leukemia. None of these children has developed malignant lymphoproliferative disease. Our data suggest that the immunological evaluation of BM in childhood is highly capable of discriminating between different malignant populations but it does not recognize malignancy and therefore supplements but cannot replace conventional methods for diagnosis.
ISSN:0888-0018
1521-0669
DOI:10.3109/08880019709030882