Effects of thidiazuron and benzyladenine on axillary shoot proliferation of three green ash (Fraxinus pennsylvanica Marsch.) clones

Mature seeds of three green ash (Fraxinus pennsylvanica Marsh) clones, SD 1009 (South Dakota origin), SD2002 (South Dakota origin), and KA2018 (Kansas origin) were cut to remove the apical portion and germinated on Murashige and Skoog (1962) salts with B5 vitamins (Gamborg et al., 1968) (MSB5) mediu...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 1997, Vol.48 (1), p.45-52
Main Authors: Kim, M.S. (Nebraska Univ., Lincoln, NE (USA). Dept. of Forestry, Fisheries and Wildlife), Schumann, C.M, Klopfenstein, N.B
Format: Article
Language:English
Subjects:
6-BENZILADENINA
6-BENZYLADENINE
AIB
benzyladenine
Biological and medical sciences
Biotechnology
clones
culture media
dose response
Eukaryotic cell cultures
FRAXINUS PENNSYLVANICA
Fundamental and applied biological sciences. Psychology
genetic variation
genotype
growth
IBA
in vitro culture
In vitro propagation: entire plant regeneration from tissues and cell cultures
indole butyric acid
methodology
Methods. Procedures. Technologies
MICROPROPAGACION
MICROPROPAGATION
murashige and skook medium
Plant cells and fungal cells
salts with b5 vitamins medium
seedlings
shoots
STEMS
TALLO
THIDIAZURON
TIDIAZURON
TIGE
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Summary:Mature seeds of three green ash (Fraxinus pennsylvanica Marsh) clones, SD 1009 (South Dakota origin), SD2002 (South Dakota origin), and KA2018 (Kansas origin) were cut to remove the apical portion and germinated on Murashige and Skoog (1962) salts with B5 vitamins (Gamborg et al., 1968) (MSB5) medium without plant growth regulators. Stable axillary shoot establishment was achieved for all three clones by subculture on MSB5 medium containing a combination of 5 micromolar thidiazuron (TDZ), 5 micromolar 6-benzyladenine (BA), and 1 micromolar indole-3-butyric acid (IBA). Following shoot establishment, axillary shoots were placed on MSB5 medium containing a single treatment of TDZ (1, 5, 10, 20, or 40 micromole) or BA (1, 5, 10, 20, 40, or 80 micromolar). Concentration of TDZ and BA significantly affected shoot biomass (total dry weight of axillary shoots), with 10 micromolar TDZ or 40 micromolar BA providing maximum shoot proliferation with all three clones. Significant clonal differences also were noted in the proliferation of axillary shoots, with clone SD1009 exhibiting the highest axillary shoot proliferation. Axillary shoots were rooted under ex vitro conditions and acclimatized to the greenhouse.
ISSN:0167-6857
1573-5044
DOI:10.1023/A:1005856720650