Purification and characterization of chaperonin 60 and heat-shock protein 70 from chromoplasts of Narcissus pseudonarcissus. Involvement of heat-shock protein 70 in a soluble protein complex containing phytoene desaturase

In chromoplast differentiation during flower formation in Narcissus pseudonarcissus, the molecular chaperones chaperonin 60 (Cpn60; alpha and beta) and heat-shock protein 70 (Hsp70) greatly increase in abundance. Both were purified and shown to be present in a functional form in chromoplasts, indica...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1996-07, Vol.111 (3), p.931-939
Main Authors: Bonk, M. (Institut fur Biologie II der Universitat, Freiburg, Germany.), Tadros, M, Vandekerckhove, J, Al-Babili, S, Beyer, P
Format: Article
Language:English
Subjects:
Antibodies
Biochemistry and Enzymology
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
CAROTENOIDE
CAROTENOIDES
Chaperonins
Chloroplasts
Chromoplasts
Citrates
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DIFERENCIACION CELULAR
DIFFERENCIATION CELLULAIRE
Enzymes
ESTRES TERMICO
ESTRUCTURA CELULAR
FLEUR
FLORES
Fundamental and applied biological sciences. Psychology
Gels
Heat shock proteins
LIASAS
LYASE
Metabolism
NARCISSUS
Nitrogen metabolism
OXIDORREDUCTASAS
OXYDOREDUCTASE
Plant physiology and development
PLASTE
PLASTIDIOS
Plastids
PROTEINAS
PROTEINE
Proteins
STRESS THERMIQUE
STRUCTURE CELLULAIRE
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Summary:In chromoplast differentiation during flower formation in Narcissus pseudonarcissus, the molecular chaperones chaperonin 60 (Cpn60; alpha and beta) and heat-shock protein 70 (Hsp70) greatly increase in abundance. Both were purified and shown to be present in a functional form in chromoplasts, indicating their requirement in the extensive structural rearrangements during the chloroplast-to-chromoplast transition. The purified proteins, sequenced N terminally and from internal peptides, showed strong homology to plastic Cpn60 and Hsp70 representatives from other plant species. During chromoplast differentiation, the carotenoid biosynthetic pathway is strongly induced. The corresponding enzymes are all nuclear encoded and form a large, soluble, hetero-oligomeric protein complex after import but prior to their membrane attachment. By immunoprecipitations we have shown that the plastic Hsp70 is a structural constituent of a soluble entity also containing phytoene desaturase
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.111.3.931