Investigation of the Effects of Solution Composition and Container Material Type on the Loss of 11-nor-Δ9-THC-9-Carboxylic Acid

The loss of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) from solution was studied using fluorescence polarization immunoassay (FPIA) technology and x-ray photoelectron spectroscopy (XPS). Several materials (glass, silylated glass, high density polyethylene, polypropylene, polystyrene...

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Bibliographic Details
Published in:Journal of analytical toxicology 1996-09, Vol.20 (5), p.291-300
Main Authors: Roth, Kenneth D.W., Siegel, Neal A., Johnson, Robert W., Litauszki, László, Salvati, Lawrence, Harrington, Charles A., Wray, Larry K.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Drug addictions
Medical sciences
Toxicology
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Summary:The loss of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) from solution was studied using fluorescence polarization immunoassay (FPIA) technology and x-ray photoelectron spectroscopy (XPS). Several materials (glass, silylated glass, high density polyethylene, polypropylene, polystyrene, polymethylmethacrylate, Teflon® and Kynar®) were studied along with three solvents (water, urine, and Abbott® cannabinoids diluent). THC-COOH losses ranging from 0 to 9.7 ng/cm2 and concentration reductions to 46% of starting values were measured. XPS indicated the presence of fluorine-labeled THC-COOH at materials surfaces. A half-life of 10 min was calculated for THC-COOH loss from urine stored in high density polyethylene at room temperature. Sample handling losses during pipetting were determined and ranged from 1.1 to 7.9 ng per aliquot. The effects of sample volume and sample handling on the THC-COOH concentrations of controls were also investigated.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/20.5.291