Genetic diversity and population structure of the secondary symbiont of tsetse flies, Sodalis glossinidius, in sleeping sickness foci in Cameroon

Previous studies have shown substantial differences in Sodalis glossinidius and trypanosome infection rates between Glossina palpalis palpalis populations from two Cameroonian foci of human African trypanosomiasis (HAT), Bipindi and Campo. We hypothesized that the geographical isolation of the two f...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2011-08, Vol.5 (8), p.e1281-e1281
Main Authors: Farikou, Oumarou, Thevenon, Sophie, Njiokou, Flobert, Allal, François, Cuny, Gérard, Geiger, Anne
Format: Article
Language:English
Subjects:
Animals
Biological diversity
Biology
Cameroon
Cluster Analysis
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Drug resistance
Enterobacteriaceae - classification
Enterobacteriaceae - genetics
Enterobacteriaceae - isolation & purification
Genetic aspects
Genetic diversity
Genetic structure
Genetic Variation
Genotypes
Glossina
Haplotypes
Insects
Investigations
Medicine
Microsatellite Repeats
Molecular Sequence Data
Phylogeography
Physiological aspects
Population
Population structure
Sequence Analysis, DNA
Sodalis glossinidius
Subpopulations
Tropical diseases
Trypanosoma
Trypanosomiasis
Tsetse Flies - microbiology
Tsetse-flies
Vector-borne diseases
Veterinary Science
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Summary:Previous studies have shown substantial differences in Sodalis glossinidius and trypanosome infection rates between Glossina palpalis palpalis populations from two Cameroonian foci of human African trypanosomiasis (HAT), Bipindi and Campo. We hypothesized that the geographical isolation of the two foci may have induced independent evolution in the two areas, resulting in the diversification of symbiont genotypes. To test this hypothesis, we investigated the symbiont genetic structure using the allelic size variation at four specific microsatellite loci. Classical analysis of molecular variance (AMOVA) and differentiation statistics revealed that most of the genetic diversity was observed among individuals within populations and frequent haplotypes were shared between populations. The structure of genetic diversity varied at different geographical scales, with almost no differentiation within the Campo HAT focus and a low but significant differentiation between the Campo and Bipindi HAT foci. The data provided new information on the genetic diversity of the secondary symbiont population revealing mild structuring. Possible interactions between S. glossinidius subpopulations and Glossina species that could favor tsetse fly infections by a given trypanosome species should be further investigated.
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0001281