Fitness of Escherichia coli during urinary tract infection requires gluconeogenesis and the TCA cycle

Microbial pathogenesis studies traditionally encompass dissection of virulence properties such as the bacterium's ability to elaborate toxins, adhere to and invade host cells, cause tissue damage, or otherwise disrupt normal host immune and cellular functions. In contrast, bacterial metabolism...

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Bibliographic Details
Published in:PLoS pathogens 2009-05, Vol.5 (5), p.e1000448-e1000448
Main Authors: Alteri, Christopher J, Smith, Sara N, Mobley, Harry L T
Format: Article
Language:English
Subjects:
Bacterial Proteins - analysis
Bacterial Proteins - urine
Biochemistry
Causes of
Citric Acid Cycle
Development and progression
Electrophoresis, Gel, Two-Dimensional
Escherichia coli
Escherichia coli - metabolism
Escherichia coli - pathogenicity
Escherichia coli - physiology
Genes
Genetic aspects
Gluconeogenesis
Humans
Infections
Infectious Diseases/Bacterial Infections
Infectious Diseases/Urological Infections
Metabolic Networks and Pathways
Microbial metabolism
Microbiology
Microbiology/Cellular Microbiology and Pathogenesis
Microbiology/Microbial Physiology and Metabolism
Physiological aspects
Proteins
Proteomics - methods
Tandem Mass Spectrometry
Urinary tract infections
Urinary Tract Infections - microbiology
Virulence (Microbiology)
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Summary:Microbial pathogenesis studies traditionally encompass dissection of virulence properties such as the bacterium's ability to elaborate toxins, adhere to and invade host cells, cause tissue damage, or otherwise disrupt normal host immune and cellular functions. In contrast, bacterial metabolism during infection has only been recently appreciated to contribute to persistence as much as their virulence properties. In this study, we used comparative proteomics to investigate the expression of uropathogenic Escherichia coli (UPEC) cytoplasmic proteins during growth in the urinary tract environment and systematic disruption of central metabolic pathways to better understand bacterial metabolism during infection. Using two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE) and tandem mass spectrometry, it was found that UPEC differentially expresses 84 cytoplasmic proteins between growth in LB medium and growth in human urine (P
ISSN:1553-7374
1553-7366
1553-7374
DOI:10.1371/journal.ppat.1000448