Rapid and Targeted Introgression of Genes into Popular Wheat Cultivars Using Marker-Assisted Background Selection

A marker-assisted background selection (MABS)-based gene introgression approach in wheat (Triticum aestivum L.) was optimized, where 97% or more of a recurrent parent genome (RPG) can be recovered in just two backcross (BC) generations. A four-step MABS method was developed based on 'Plabsim�...

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Published in:PloS one 2009-06, Vol.4 (6), p.e5752-e5752
Main Authors: Randhawa, Harpinder S, Mutti, Jasdeep S, Kidwell, Kim, Morris, Craig F, Chen, Xianming, Gill, Kulvinder S
Format: Article
Language:English
Subjects:
Agronomy
backcrossing
Biotechnology
Biotechnology/Plant Biotechnology
Carriers
Chromosome Mapping
Chromosomes
Chromosomes - ultrastructure
Computer Simulation
Crosses, Genetic
Cultivars
disease resistance
Empirical analysis
Genes
Genes, Plant
Genetic aspects
Genetic Markers
Genetic research
Genetics and Genomics
Genetics and Genomics/Bioinformatics
genome
Genome, Plant
Genomes
Genomics
Genotype
Heterozygote
introgression
marker-assisted background selection
marker-assisted selection
Markers
Mathematical models
Models, Genetic
Optimization
Oryza
Plant Biology/Agricultural Biotechnology
plant breeding
Plant Diseases - genetics
Plant pathology
Population
Probability
Progeny
Puccinia
Puccinia recondita
Recombinants
Recombination, Genetic
Rice
rust diseases
Seedlings
Simulation
simulation models
Soil sciences
Spring wheat
Stripe rust
Triticum - genetics
Triticum aestivum
Triticum dicoccum
Wheat
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cited_by cdi_FETCH-LOGICAL-c717t-a3be69b975c064646a0ae400dd6de7299a2dfefbf842b730d92d0051b00bcd2c3
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container_issue 6
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container_title PloS one
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creator Randhawa, Harpinder S
Mutti, Jasdeep S
Kidwell, Kim
Morris, Craig F
Chen, Xianming
Gill, Kulvinder S
description A marker-assisted background selection (MABS)-based gene introgression approach in wheat (Triticum aestivum L.) was optimized, where 97% or more of a recurrent parent genome (RPG) can be recovered in just two backcross (BC) generations. A four-step MABS method was developed based on 'Plabsim' computer simulations and wheat genome structure information. During empirical optimization of this method, double recombinants around the target gene were selected in a step-wise fashion during the two BC cycles followed by selection for recurrent parent genotype on non-carrier chromosomes. The average spacing between carrier chromosome markers was
doi_str_mv 10.1371/journal.pone.0005752
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Field evaluation results from 17 locations indicated that the MABS-derived line was either equal or superior to the recurrent parent for the tested agronomic characteristics. 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Field evaluation results from 17 locations indicated that the MABS-derived line was either equal or superior to the recurrent parent for the tested agronomic characteristics. Based on these results, MABS is recommended as a strategy for rapidly introgressing a targeted gene into a wheat genotype in just two backcross generations while recovering 97% or more of the recurrent parent genotype.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>19484121</pmid><doi>10.1371/journal.pone.0005752</doi><tpages>e5752</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
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source Open Access: PubMed Central; ProQuest - Publicly Available Content Database
subjects Agronomy
backcrossing
Biotechnology
Biotechnology/Plant Biotechnology
Carriers
Chromosome Mapping
Chromosomes
Chromosomes - ultrastructure
Computer Simulation
Crosses, Genetic
Cultivars
disease resistance
Empirical analysis
Genes
Genes, Plant
Genetic aspects
Genetic Markers
Genetic research
Genetics and Genomics
Genetics and Genomics/Bioinformatics
genome
Genome, Plant
Genomes
Genomics
Genotype
Heterozygote
introgression
marker-assisted background selection
marker-assisted selection
Markers
Mathematical models
Models, Genetic
Optimization
Oryza
Plant Biology/Agricultural Biotechnology
plant breeding
Plant Diseases - genetics
Plant pathology
Population
Probability
Progeny
Puccinia
Puccinia recondita
Recombinants
Recombination, Genetic
Rice
rust diseases
Seedlings
Simulation
simulation models
Soil sciences
Spring wheat
Stripe rust
Triticum - genetics
Triticum aestivum
Triticum dicoccum
Wheat
title Rapid and Targeted Introgression of Genes into Popular Wheat Cultivars Using Marker-Assisted Background Selection
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