Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis

Little is known about short-term bacterial fluctuations in the human vagina. This study used PCR to assess the variability in concentrations of key vaginal bacteria in healthy women and the immediate response to antibiotic treatment in women with bacterial vaginosis (BV). Twenty-two women assessed f...

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Bibliographic Details
Published in:PloS one 2010-04, Vol.5 (4), p.e10197-e10197
Main Authors: Srinivasan, Sujatha, Liu, Congzhou, Mitchell, Caroline M, Fiedler, Tina L, Thomas, Katherine K, Agnew, Kathy J, Marrazzo, Jeanne M, Fredricks, David N
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Antiprotozoan agents
Bacteria
Bacterial vaginosis
Cancer
Deoxyribonucleic acid
DNA
Family medical history
Female
Gardnerella vaginalis
Gardnerella vaginalis - isolation & purification
Genetic testing
Gynecology
Humans
Immunization
Infectious diseases
Infectious Diseases/Bacterial Infections
Infectious Diseases/Gynecologic Infections
Infectious Diseases/Sexually Transmitted Diseases
Lactobacilli
Lactobacillus
Lactobacillus - isolation & purification
Medical research
Menstrual Cycle
Menstruation
Metronidazole
Metronidazole - administration & dosage
Microbial drug resistance
Microbiology/Medical Microbiology
Microbiota (Symbiotic organisms)
Obstetrics
Phylogenetics
Polymerase Chain Reaction
Recurrence
Sexually transmitted diseases
STD
Temporal variability
Vaccines
Vagina
Vaginosis
Vaginosis, Bacterial - microbiology
Variability
Women's health
Women's Health/Gynecologic Inflammation and Infections
Women's Health/Sexually Transmitted Diseases
Womens health
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Summary:Little is known about short-term bacterial fluctuations in the human vagina. This study used PCR to assess the variability in concentrations of key vaginal bacteria in healthy women and the immediate response to antibiotic treatment in women with bacterial vaginosis (BV). Twenty-two women assessed for BV using Amsel's criteria were evaluated daily for 7 or 14 days, then at 2, 3 and 4 weeks, using a panel of 11 bacterium-specific quantitative PCR assays. Participants with BV were treated with 5 days of intravaginal metronidazole. Participants without BV had vaginal biotas dominated by lactobacilli, whose levels fluctuated with menses. With onset of menstruation, quantities of Lactobacillus jensenii and Lactobacillus crispatus decreased and were found to be inversely related to Gardnerella vaginalis concentrations (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0010197