Real-time dynamic imaging of virus distribution in vivo

The distribution of viruses and gene therapy vectors is difficult to assess in a living organism. For instance, trafficking in murine models can usually only be assessed after sacrificing the animal for tissue sectioning or extraction. These assays are laborious requiring whole animal sectioning to...

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Bibliographic Details
Published in:PloS one 2011-02, Vol.6 (2), p.e17076-e17076
Main Authors: Hofherr, Sean E, Adams, Kristen E, Chen, Christopher Y, May, Shannon, Weaver, Eric A, Barry, Michael A
Format: Article
Language:English
Subjects:
Adenoviridae - metabolism
Adenoviridae - physiology
Adenoviruses
Animal care
Animal models
Animals
Biology
Breast cancer
Cytomegalovirus
Disseminated infection
Distributing
Dyes
Expression vectors
Female
Fluorescent Dyes - metabolism
Gene expression
Gene therapy
Genetic vectors
Health aspects
I.R. radiation
Indoles - metabolism
Infections
Infectious diseases
Infrared imaging
Infrared Rays
Injection
Injections
Internal medicine
Intravenous administration
Jugular vein
Laboratory animals
Localization
Medical imaging
Medicine
Mice
Molecular Imaging - methods
Proteins
Real time
Sectioning
Time Factors
Tomography
Tracking
Viral infections
Viral proteins
Viral Tropism
Viruses
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Description
Summary:The distribution of viruses and gene therapy vectors is difficult to assess in a living organism. For instance, trafficking in murine models can usually only be assessed after sacrificing the animal for tissue sectioning or extraction. These assays are laborious requiring whole animal sectioning to ascertain tissue localization. They also obviate the ability to perform longitudinal or kinetic studies in one animal. To track viruses after systemic infection, we have labeled adenoviruses with a near-infrared (NIR) fluorophore and imaged these after intravenous injection in mice. Imaging was able to track and quantitate virus particles entering the jugular vein simultaneous with injection, appearing in the heart within 500 milliseconds, distributing in the bloodstream and throughout the animal within 7 seconds, and that the bulk of virus distribution was essentially complete within 3 minutes. These data provide the first in vivo real-time tracking of the rapid initial events of systemic virus infection.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0017076