False-positive human immunodeficiency virus enzyme immunoassay results in pregnant women

Examine whether false-positive HIV enzyme immunoassay (EIA) test results occur more frequently among pregnant women than among women who are not pregnant and men (others). To obtain a large number of pregnant women and others tested for HIV, we identified specimens tested at a national laboratory us...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2011-01, Vol.6 (1), p.e16538-e16538
Main Authors: Wesolowski, Laura G, Delaney, Kevin P, Lampe, Margaret A, Nesheim, Steven R
Format: Article
Language:English
Subjects:
Acquired immune deficiency syndrome
Adult
AIDS
Antiretroviral drugs
Biology
Blotting, Western
Comparative analysis
Confidence intervals
Disease prevention
Enzyme immunoassay
False Positive Reactions
Female
Health risk assessment
HIV
HIV - isolation & purification
HIV Infections - diagnosis
HIV Infections - epidemiology
HIV tests
Human immunodeficiency virus
Human immunodeficiency virus 1
Human immunodeficiency virus 2
Humans
Immunoassay
Immunoassay enzymes
Immunoenzyme Techniques - methods
Immunoenzyme Techniques - standards
Laboratories
Medicine
Pregnancy
Pregnancy Complications - diagnosis
Pregnancy Complications - virology
Pregnant women
Prevalence
Viruses
Womens health
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Examine whether false-positive HIV enzyme immunoassay (EIA) test results occur more frequently among pregnant women than among women who are not pregnant and men (others). To obtain a large number of pregnant women and others tested for HIV, we identified specimens tested at a national laboratory using Genetic Systems HIV-1/HIV-2 Plus O EIA from July 2007 to June 2008. Specimens with EIA repeatedly reactive and Western blot-negative or indeterminate results were considered EIA false-positive. We compared the false-positive rate among uninfected pregnant women and others, adjusting for HIV prevalence. Among all reactive EIAs, we evaluated the proportion of false-positives, positive predictive value (PPV), and Western blot bands among indeterminates, by pregnancy status. HIV prevalence was 0.06% among 921,438 pregnant women and 1.34% among 1,103,961 others. The false-positive rate was lower for pregnant women than others (0.14% vs. 0.21%, odds ratio 0.65 [95% confidence interval 0.61, 0.70]). Pregnant women with reactive EIAs were more likely than others (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0016538