PS integrins and laminins: key regulators of cell migration during Drosophila embryogenesis

During embryonic development, there are numerous cases where organ or tissue formation depends upon the migration of primordial cells. In the Drosophila embryo, the visceral mesoderm (vm) acts as a substrate for the migration of several cell populations of epithelial origin, including the endoderm,...

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Published in:PloS one 2011-09, Vol.6 (9), p.e23893-e23893
Main Authors: Urbano, Jose M, Domínguez-Giménez, Paloma, Estrada, Beatriz, Martín-Bermudo, María D
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Biology
Cell adhesion & migration
Cell migration
Cell Movement
Drosophila
Drosophila - embryology
Drosophila - genetics
Drosophila - metabolism
Drosophila melanogaster
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - embryology
Embryo, Nonmammalian - metabolism
Embryogenesis
Embryonic development
Embryonic growth stage
Embryos
Endoderm
Extracellular matrix
Gene expression
Glands
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
In Situ Hybridization
Insects
Integrins
Integrins - genetics
Integrins - metabolism
Kinases
Laminin
Laminin - genetics
Laminin - metabolism
Mammals
Mesoderm
Mesoderm - cytology
Mesoderm - embryology
Mesoderm - metabolism
Microscopy, Confocal
Morphogenesis
Mutation
Presenilin 1
Presenilin 2
Regulators
Salivary glands
Time-Lapse Imaging
Trachea
Vertebrates
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container_issue 9
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creator Urbano, Jose M
Domínguez-Giménez, Paloma
Estrada, Beatriz
Martín-Bermudo, María D
description During embryonic development, there are numerous cases where organ or tissue formation depends upon the migration of primordial cells. In the Drosophila embryo, the visceral mesoderm (vm) acts as a substrate for the migration of several cell populations of epithelial origin, including the endoderm, the trachea and the salivary glands. These migratory processes require both integrins and laminins. The current model is that αPS1βPS (PS1) and/or αPS3βPS (PS3) integrins are required in migrating cells, whereas αPS2βPS (PS2) integrin is required in the vm, where it performs an as yet unidentified function. Here, we show that PS1 integrins are also required for the migration over the vm of cells of mesodermal origin, the caudal visceral mesoderm (CVM). These results support a model in which PS1 might have evolved to acquire the migratory function of integrins, irrespective of the origin of the tissue. This integrin function is highly specific and its specificity resides mainly in the extracellular domain. In addition, we have identified the Laminin α1,2 trimer, as the key extracellular matrix (ECM) component regulating CVM migration. Furthermore, we show that, as it is the case in vertebrates, integrins, and specifically PS2, contributes to CVM movement by participating in the correct assembly of the ECM that serves as tracks for migration.
doi_str_mv 10.1371/journal.pone.0023893
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subjects Animals
Animals, Genetically Modified
Biology
Cell adhesion & migration
Cell migration
Cell Movement
Drosophila
Drosophila - embryology
Drosophila - genetics
Drosophila - metabolism
Drosophila melanogaster
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - embryology
Embryo, Nonmammalian - metabolism
Embryogenesis
Embryonic development
Embryonic growth stage
Embryos
Endoderm
Extracellular matrix
Gene expression
Glands
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
In Situ Hybridization
Insects
Integrins
Integrins - genetics
Integrins - metabolism
Kinases
Laminin
Laminin - genetics
Laminin - metabolism
Mammals
Mesoderm
Mesoderm - cytology
Mesoderm - embryology
Mesoderm - metabolism
Microscopy, Confocal
Morphogenesis
Mutation
Presenilin 1
Presenilin 2
Regulators
Salivary glands
Time-Lapse Imaging
Trachea
Vertebrates
title PS integrins and laminins: key regulators of cell migration during Drosophila embryogenesis
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