Quantitative high-resolution genomic analysis of single cancer cells

During cancer progression, specific genomic aberrations arise that can determine the scope of the disease and can be used as predictive or prognostic markers. The detection of specific gene amplifications or deletions in single blood-borne or disseminated tumour cells that may give rise to the devel...

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Bibliographic Details
Published in:PloS one 2011-11, Vol.6 (11), p.e26362-e26362
Main Authors: Hannemann, Juliane, Meyer-Staeckling, Sönke, Kemming, Dirk, Alpers, Iris, Joosse, Simon A, Pospisil, Heike, Kurtz, Stefan, Görndt, Jennifer, Püschel, Klaus, Riethdorf, Sabine, Pantel, Klaus, Brandt, Burkhard
Format: Article
Language:English
Subjects:
Aberration
Analysis
Bioinformatics
Biology
Bone marrow
Breast cancer
Cancer
Cancer cells
Cancer genetics
Cancer metastasis
Cancer therapies
Cell Line, Tumor
Chemotherapy
Chromosomes
Colorectal cancer
Comparative Genomic Hybridization
Copy number
Cytogenetics
Deoxyribonucleic acid
Development and progression
DNA
Epidermal growth factor
Epidermal growth factor receptors
Esophageal cancer
Genes
Genetic Heterogeneity
Genetic research
Genomes
Genomic analysis
Genomics
Genomics - methods
High resolution
Humans
Hybridization
Kinases
Legal medicine
Mastectomy
Medicine
Metastases
Neoplasms - blood
Neoplasms - genetics
Neoplasms - pathology
Neuroblastoma
Polymerase Chain Reaction
Prognosis
Quantitative analysis
Quantitative genetics
Receptor, Epidermal Growth Factor - genetics
Single-Cell Analysis - methods
Stem cells
Tiling
Tumors
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Summary:During cancer progression, specific genomic aberrations arise that can determine the scope of the disease and can be used as predictive or prognostic markers. The detection of specific gene amplifications or deletions in single blood-borne or disseminated tumour cells that may give rise to the development of metastases is of great clinical interest but technically challenging. In this study, we present a method for quantitative high-resolution genomic analysis of single cells. Cells were isolated under permanent microscopic control followed by high-fidelity whole genome amplification and subsequent analyses by fine tiling array-CGH and qPCR. The assay was applied to single breast cancer cells to analyze the chromosomal region centred by the therapeutical relevant EGFR gene. This method allows precise quantitative analysis of copy number variations in single cell diagnostics.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0026362