tRNA gene identity affects nuclear positioning

The three-dimensional organization of genomes is dynamic and plays a critical role in the regulation of cellular development and phenotypes. Here we use proximity-based ligation methods (i.e. chromosome conformation capture [3C] and circularized chromosome confrmation capture [4C]) to explore the sp...

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Bibliographic Details
Published in:PloS one 2011-12, Vol.6 (12), p.e29267-e29267
Main Authors: Rodley, Chris D M, Pai, Dave A, Mills, Tyrone A, Engelke, David R, O'Sullivan, Justin M
Format: Article
Language:English
Subjects:
Analysis
Biology
Biosynthesis
Cell cycle
Cell Nucleolus - genetics
Cell Nucleus - genetics
Chromosomes
Chromosomes, Fungal - genetics
Conformation
Deoxyribonucleic acid
DNA
Gene expression
Gene loci
Genes
Genes, Fungal - genetics
Genetic Loci - genetics
Genomes
Genomics
Leucine
Localization
Lysine
Lysine - metabolism
Microscopy
Molecular biology
Mutation
Nuclei
RNA polymerase
RNA, Transfer - genetics
RNA, Transfer - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Three dimensional models
Transfer RNA
tRNA Leu
tRNA Lys
Yeast
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Summary:The three-dimensional organization of genomes is dynamic and plays a critical role in the regulation of cellular development and phenotypes. Here we use proximity-based ligation methods (i.e. chromosome conformation capture [3C] and circularized chromosome confrmation capture [4C]) to explore the spatial organization of tRNA genes and their locus-specific interactions with the ribosomal DNA. Directed replacement of one lysine and two leucine tRNA loci shows that tRNA spatial organization depends on both tRNA coding sequence identity and the surrounding chromosomal loci. These observations support a model whereby the three-dimensional, spatial organization of tRNA loci within the nucleus utilizes tRNA gene-specific signals to affect local interactions, though broader organization of chromosomal regions are determined by factors outside the tRNA genes themselves.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0029267