Gain-of-function screen for genes that affect Drosophila muscle pattern formation

This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsiv...

Full description

Saved in:
Bibliographic Details
Published in:PLoS genetics 2005-10, Vol.1 (4), p.e55-e55
Main Authors: Staudt, Nicole, Molitor, Andreas, Somogyi, Kalman, Mata, Juan, Curado, Silvia, Eulenberg, Karsten, Meise, Martin, Siegmund, Thomas, Häder, Thomas, Hilfiker, Andres, Brönner, Günter, Ephrussi, Anne, Rørth, Pernille, Cohen, Stephen M, Fellert, Sonja, Chung, Ho-Ryun, Piepenburg, Olaf, Schäfer, Ulrich, Jäckle, Herbert, Vorbrüggen, Gerd
Format: Article
Language:English
Subjects:
Animals
Body Patterning
Cell Cycle
Cell Movement
Cytoskeleton - metabolism
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Gene Expression Regulation, Developmental
Genes
Genes, Insect
Genetic Techniques
Genetics
Genomes
Heparan sulfate
Kinases
Library collections
Migration
Muscle Fibers, Skeletal - metabolism
Muscles - cytology
Muscles - metabolism
Muscles - pathology
Ubiquitin - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.0010055