Male microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation

Patients with end stage renal diseases (ESRD) are generally tested for donor chimerism after kidney transplantation for tolerance mechanism purposes. But, to our knowledge, no data are available on natural and/or iatrogenic microchimerism (Mc), deriving from pregnancy and/or blood transfusion, acqui...

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Bibliographic Details
Published in:PloS one 2012-03, Vol.7 (3), p.e32248-e32248
Main Authors: Albano, Laetitia, Rak, Justyna M, Azzouz, Doua F, Cassuto-Viguier, Elisabeth, Gugenheim, Jean, Lambert, Nathalie C
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Biology
Biopsy
Blood
Blood Transfusion
Bone marrow
Breast cancer
Case-Control Studies
Chimerism
Chimerism - statistics & numerical data
Chronic kidney failure
Data processing
Deoxyribonucleic acid
Diabetes
DNA
Female
Females
Genetic testing
Genomes
Genomics
Humans
Hypertension
Immunological tolerance
Kidney - pathology
Kidney diseases
Kidney Failure, Chronic - blood
Kidney Failure, Chronic - genetics
Kidney Failure, Chronic - pathology
Kidney Failure, Chronic - therapy
Kidney Transplantation
Kidney transplants
Leukocytes (mononuclear)
Leukocytes, Mononuclear - metabolism
Lupus
Lymphatic system
Male
Medicine
Middle Aged
Organ transplantation
Peripheral blood mononuclear cells
Polymerase Chain Reaction
Pregnancy
Rheumatoid arthritis
Rodents
Scleroderma
Thyroid gland
Time Factors
Transfusion
Transplantation
Transplants & implants
Women's health
Womens health
Y chromosomes
Young Adult
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Summary:Patients with end stage renal diseases (ESRD) are generally tested for donor chimerism after kidney transplantation for tolerance mechanism purposes. But, to our knowledge, no data are available on natural and/or iatrogenic microchimerism (Mc), deriving from pregnancy and/or blood transfusion, acquired prior to transplantation. In this context, we tested the prevalence of male Mc using a real time PCR assay for DYS14, a Y-chromosome specific sequence, in peripheral blood mononuclear cells (PBMC) from 55 women with ESRD, prior to their first kidney transplantation, and compared them with results from 82 healthy women. Male Mc was also quantified in 5 native kidney biopsies obtained two to four years prior to blood testing and in PBMC from 8 women collected after female kidney transplantation, several years after the initial blood testing. Women with ESRD showed statistically higher frequencies (62%) and quantities (98 genome equivalent cells per million of host cells, gEq/M) of male Mc in their PBMC than healthy women (16% and 0.3 gEq/M, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0032248