Supplementing with non-glycoside hydrolase proteins enhances enzymatic deconstruction of plant biomass

• Published in: PloS one 2012-08, Vol.7 (8), p.e43828-e43828
• Main Authors: Su, Xiaoyun, Zhang, Jing, Mackie, Roderick I, Cann, Isaac K O
• Format: Article
• Language: English
• Subjects: Animal sciences; Animals; Archaeal Proteins - metabolism; Bacterial Proteins - metabolism; Biodiesel fuels; Biofuels; Biology; Biomass; Caldicellulosiruptor bescii; Cattle; Cell Wall - metabolism; Cell walls; Cellulase; Cellulase - metabolism; Cellulose; Cellulose - metabolism; Deconstruction; Enzymatic activity; Enzyme Stability; Enzymes; Euryarchaeota; Fermentation; Glycoside hydrolase; Glycoside Hydrolases - metabolism; Growth hormone; Heat shock proteins; Heat-Shock Proteins - metabolism; High temperature; Histones - metabolism; Hydrolase; Hydrolysis; Lignocellulose; Oligosaccharides - metabolism; Plant biomass; Plants - metabolism; Polypeptides; Polysaccharides; Proteins; Ribonuclease, Pancreatic - metabolism; Saccharides; Shaking; Spectrum analysis; Studies; Substrates; Temperature; Thermal stability; Thermoanaerobacterium; Trichoderma reesei
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0043828

The glycoside hydrolases (GH) of Caldicellulosiruptor bescii are thermophilic enzymes, and therefore they can hydrolyze plant cell wall polysaccharides at high temperatures. Analyses of two C. bescii glycoside hydrolases, CbCelA-TM1 and CbXyn10A with cellulase and endoxylanase activity, respectively, demonstrated that each enzyme is highly thermostable under static incubation at 70°C. Both enzymes, however, rapidly lost their enzymatic activities when incubated at 70°C with end-over-end shaking. Since crowding conditions, even at low protein concentrations, seem to influence enzymatic properties, three non-glycoside hydrolase proteins were tested for their capacity to stabilize the thermophilic proteins at high temperatures. The three proteins investigated were a small heat shock protein CbHsp18 from C. bescii, a histone MkHistone1 from Methanopyrus kandleri, and bovine RNase A, from a commercial source. Fascinatingly, each of these proteins increased the thermostability of the glycoside hydrolases at 70°C during end-over-end shaking incubation, and this property translated into increases in hydrolysis of several substrates including the bioenergy feedstock Miscanthus. Furthermore, MkHistone1 and RNase A also altered the initial products released from the cello-oligosaccharide cellopentaose during hydrolysis with the cellodextrinase CbCdx1A, which further demonstrated the capacity of the three non-GH proteins to influence hydrolysis of substrates by the thermophilic glycoside hydrolases. The non-GH proteins used in the present report were small proteins derived from each of the three lineages of life, and therefore expand the space from which different polypeptides can be tested for their influence on plant cell wall hydrolysis, a critical step in the emerging biofuel industry.