Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis

Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain re...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2012-09, Vol.7 (9), p.e44287
Main Authors: Nogues, Claude, Leh, Hervé, Lautru, Joseph, Delelis, Olivier, Buckle, Malcolm
Format: Article
Language:English
Subjects:
Adsorption
Analysis
Analytical chemistry
Antifouling
Antifouling substances
Binding
Biofouling - prevention & control
Biology
Biomolecules
Biosensors
Biotechnology
Chemical properties
Deoxyribonucleic acid
Detection equipment
DNA
DNA - metabolism
Hybridization
Immobilized Nucleic Acids - metabolism
Integrases - metabolism
Kinetics
Life Sciences
Ligands
Macromolecules
Models, Molecular
Monolayers
Physical chemistry
Protein Binding
Proteins
Quantitative analysis
Retroviridae - metabolism
Self assembly
Self-assembled monolayers
Surface plasmon resonance
Surface Plasmon Resonance - methods
Surface Properties
Time Factors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c726t-56fe5c0739db466a7e34f804840ecacacf655570723a31399a3c8d535cbcb91a3
cites
container_end_page
container_issue 9
container_start_page e44287
container_title PloS one
container_volume 7
creator Nogues, Claude
Leh, Hervé
Lautru, Joseph
Delelis, Olivier
Buckle, Malcolm
description Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated.
doi_str_mv 10.1371/journal.pone.0044287
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1326545281</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A543316458</galeid><doaj_id>oai_doaj_org_article_b72d5d13c22c435aae6484bc149c8035</doaj_id><sourcerecordid>A543316458</sourcerecordid><originalsourceid>FETCH-LOGICAL-c726t-56fe5c0739db466a7e34f804840ecacacf655570723a31399a3c8d535cbcb91a3</originalsourceid><addsrcrecordid>eNqNk9uK2zAQhk1p6R7aNyitobCwF0l1tn1TCMu2Gwgs9HQrxrKUKDhSVrJD9-2rNN4QlxaKLiRmvn9mNNJk2RuMppgW-MPa98FBO916p6cIMUbK4ll2jitKJoIg-vzkfJZdxLhGiNNSiJfZGSFVyVhZnGdwa4xVVrsuB9dZ4_vWumUe-2BA6dz4kD_0e08Hnd3po2PbQtx4lwcdvQOXLDVE3eS19VG7mGSQinuMNr7KXhhoo3497JfZ90-3327uJov7z_Ob2WKiCiK6CRdGc4UKWjU1EwIKTZkpESsZ0grSMoJzXqCCUKCYVhVQVTacclWrusJAL7N3h7jb1kc5dCdKTIngjJMSJ2J-IBoPa7kNdgPhUXqw8rfBh6WE0FnValkXpOENpooQxSgH0CJVUivMKlUiylOsj0O2vt7oRqUGBmhHQcceZ1dy6XeSMoYYZSnA9SHA6g_Z3Wwh9zZEqCg5Lnb7wt8PyYJ_6HXs_nG9gVpCuoF1xqfEamOjkjPOKMWC8TJR079QaTV6Y1X6S8Ym-0hwPRIkptM_uyX0Mcr51y__z97_GLNXJ-xKQ9utom_7znoXxyA7gCr4GIM2x3ZhJPej8NQNuR8FOYxCkr09faGj6Onv01-_ZQRI</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1326545281</pqid></control><display><type>article</type><title>Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Nogues, Claude ; Leh, Hervé ; Lautru, Joseph ; Delelis, Olivier ; Buckle, Malcolm</creator><contributor>Kellermayer, Miklos S.</contributor><creatorcontrib>Nogues, Claude ; Leh, Hervé ; Lautru, Joseph ; Delelis, Olivier ; Buckle, Malcolm ; Kellermayer, Miklos S.</creatorcontrib><description>Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0044287</identifier><identifier>PMID: 22984487</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adsorption ; Analysis ; Analytical chemistry ; Antifouling ; Antifouling substances ; Binding ; Biofouling - prevention &amp; control ; Biology ; Biomolecules ; Biosensors ; Biotechnology ; Chemical properties ; Deoxyribonucleic acid ; Detection equipment ; DNA ; DNA - metabolism ; Hybridization ; Immobilized Nucleic Acids - metabolism ; Integrases - metabolism ; Kinetics ; Life Sciences ; Ligands ; Macromolecules ; Models, Molecular ; Monolayers ; Physical chemistry ; Protein Binding ; Proteins ; Quantitative analysis ; Retroviridae - metabolism ; Self assembly ; Self-assembled monolayers ; Surface plasmon resonance ; Surface Plasmon Resonance - methods ; Surface Properties ; Time Factors</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e44287</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Nogues et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2012 Nogues et al 2012 Nogues et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c726t-56fe5c0739db466a7e34f804840ecacacf655570723a31399a3c8d535cbcb91a3</citedby><orcidid>0000-0001-7596-3467</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1326545281/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1326545281?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22984487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02368517$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Kellermayer, Miklos S.</contributor><creatorcontrib>Nogues, Claude</creatorcontrib><creatorcontrib>Leh, Hervé</creatorcontrib><creatorcontrib>Lautru, Joseph</creatorcontrib><creatorcontrib>Delelis, Olivier</creatorcontrib><creatorcontrib>Buckle, Malcolm</creatorcontrib><title>Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated.</description><subject>Adsorption</subject><subject>Analysis</subject><subject>Analytical chemistry</subject><subject>Antifouling</subject><subject>Antifouling substances</subject><subject>Binding</subject><subject>Biofouling - prevention &amp; control</subject><subject>Biology</subject><subject>Biomolecules</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemical properties</subject><subject>Deoxyribonucleic acid</subject><subject>Detection equipment</subject><subject>DNA</subject><subject>DNA - metabolism</subject><subject>Hybridization</subject><subject>Immobilized Nucleic Acids - metabolism</subject><subject>Integrases - metabolism</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Ligands</subject><subject>Macromolecules</subject><subject>Models, Molecular</subject><subject>Monolayers</subject><subject>Physical chemistry</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Quantitative analysis</subject><subject>Retroviridae - metabolism</subject><subject>Self assembly</subject><subject>Self-assembled monolayers</subject><subject>Surface plasmon resonance</subject><subject>Surface Plasmon Resonance - methods</subject><subject>Surface Properties</subject><subject>Time Factors</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9uK2zAQhk1p6R7aNyitobCwF0l1tn1TCMu2Gwgs9HQrxrKUKDhSVrJD9-2rNN4QlxaKLiRmvn9mNNJk2RuMppgW-MPa98FBO916p6cIMUbK4ll2jitKJoIg-vzkfJZdxLhGiNNSiJfZGSFVyVhZnGdwa4xVVrsuB9dZ4_vWumUe-2BA6dz4kD_0e08Hnd3po2PbQtx4lwcdvQOXLDVE3eS19VG7mGSQinuMNr7KXhhoo3497JfZ90-3327uJov7z_Ob2WKiCiK6CRdGc4UKWjU1EwIKTZkpESsZ0grSMoJzXqCCUKCYVhVQVTacclWrusJAL7N3h7jb1kc5dCdKTIngjJMSJ2J-IBoPa7kNdgPhUXqw8rfBh6WE0FnValkXpOENpooQxSgH0CJVUivMKlUiylOsj0O2vt7oRqUGBmhHQcceZ1dy6XeSMoYYZSnA9SHA6g_Z3Wwh9zZEqCg5Lnb7wt8PyYJ_6HXs_nG9gVpCuoF1xqfEamOjkjPOKMWC8TJR079QaTV6Y1X6S8Ym-0hwPRIkptM_uyX0Mcr51y__z97_GLNXJ-xKQ9utom_7znoXxyA7gCr4GIM2x3ZhJPej8NQNuR8FOYxCkr09faGj6Onv01-_ZQRI</recordid><startdate>20120912</startdate><enddate>20120912</enddate><creator>Nogues, Claude</creator><creator>Leh, Hervé</creator><creator>Lautru, Joseph</creator><creator>Delelis, Olivier</creator><creator>Buckle, Malcolm</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-7596-3467</orcidid></search><sort><creationdate>20120912</creationdate><title>Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis</title><author>Nogues, Claude ; Leh, Hervé ; Lautru, Joseph ; Delelis, Olivier ; Buckle, Malcolm</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c726t-56fe5c0739db466a7e34f804840ecacacf655570723a31399a3c8d535cbcb91a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adsorption</topic><topic>Analysis</topic><topic>Analytical chemistry</topic><topic>Antifouling</topic><topic>Antifouling substances</topic><topic>Binding</topic><topic>Biofouling - prevention &amp; control</topic><topic>Biology</topic><topic>Biomolecules</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemical properties</topic><topic>Deoxyribonucleic acid</topic><topic>Detection equipment</topic><topic>DNA</topic><topic>DNA - metabolism</topic><topic>Hybridization</topic><topic>Immobilized Nucleic Acids - metabolism</topic><topic>Integrases - metabolism</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Ligands</topic><topic>Macromolecules</topic><topic>Models, Molecular</topic><topic>Monolayers</topic><topic>Physical chemistry</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Quantitative analysis</topic><topic>Retroviridae - metabolism</topic><topic>Self assembly</topic><topic>Self-assembled monolayers</topic><topic>Surface plasmon resonance</topic><topic>Surface Plasmon Resonance - methods</topic><topic>Surface Properties</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nogues, Claude</creatorcontrib><creatorcontrib>Leh, Hervé</creatorcontrib><creatorcontrib>Lautru, Joseph</creatorcontrib><creatorcontrib>Delelis, Olivier</creatorcontrib><creatorcontrib>Buckle, Malcolm</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints Resource Center</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Databases</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nogues, Claude</au><au>Leh, Hervé</au><au>Lautru, Joseph</au><au>Delelis, Olivier</au><au>Buckle, Malcolm</au><au>Kellermayer, Miklos S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-09-12</date><risdate>2012</risdate><volume>7</volume><issue>9</issue><spage>e44287</spage><pages>e44287-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22984487</pmid><doi>10.1371/journal.pone.0044287</doi><tpages>e44287</tpages><orcidid>https://orcid.org/0000-0001-7596-3467</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2012-09, Vol.7 (9), p.e44287
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1326545281
source Publicly Available Content Database; PubMed Central
subjects Adsorption
Analysis
Analytical chemistry
Antifouling
Antifouling substances
Binding
Biofouling - prevention & control
Biology
Biomolecules
Biosensors
Biotechnology
Chemical properties
Deoxyribonucleic acid
Detection equipment
DNA
DNA - metabolism
Hybridization
Immobilized Nucleic Acids - metabolism
Integrases - metabolism
Kinetics
Life Sciences
Ligands
Macromolecules
Models, Molecular
Monolayers
Physical chemistry
Protein Binding
Proteins
Quantitative analysis
Retroviridae - metabolism
Self assembly
Self-assembled monolayers
Surface plasmon resonance
Surface Plasmon Resonance - methods
Surface Properties
Time Factors
title Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T21%3A19%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Efficient%20antifouling%20surface%20for%20quantitative%20surface%20plasmon%20resonance%20based%20biosensor%20analysis&rft.jtitle=PloS%20one&rft.au=Nogues,%20Claude&rft.date=2012-09-12&rft.volume=7&rft.issue=9&rft.spage=e44287&rft.pages=e44287-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0044287&rft_dat=%3Cgale_plos_%3EA543316458%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c726t-56fe5c0739db466a7e34f804840ecacacf655570723a31399a3c8d535cbcb91a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1326545281&rft_id=info:pmid/22984487&rft_galeid=A543316458&rfr_iscdi=true