Two wheat glutathione peroxidase genes whose products are located in chloroplasts improve salt and H2O2 tolerances in Arabidopsis

• Published in: PloS one 2013-10, Vol.8 (10), p.e73989
• Main Authors: Zhai, Chao-Zeng, Zhao, Lei, Yin, Li-Juan, Chen, Ming, Wang, Qing-Yu, Li, Lian-Cheng, Xu, Zhao-Shi, Ma, You-Zhi
• Format: Article
• Language: English
• Subjects: Abscisic acid; Agriculture; Alcohol; Amino Acid Sequence; Antioxidants; Apoptosis; Arabidopsis; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - physiology; Barley; Biology; Butyl hydroperoxide; Chloroplasts; Chloroplasts - metabolism; Crop science; Damage accumulation; Drought; Enzymes; Fluorescence; Gene expression; Gene sequencing; Genes; Glutathione; Glutathione peroxidase; Glutathione Peroxidase - chemistry; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Intracellular Space - metabolism; Laboratories; Molecular Sequence Data; Organic hydroperoxide; Oxidative stress; Oxygen; Pathogens; Peroxidase; Plant sciences; Plants, Genetically Modified; Polymerase chain reaction; Protein Transport; Proteins; Reactive oxygen species; Rodents; Salts; Seedlings; Signal transduction; Signaling; Sodium Chloride - pharmacology; Stress, Physiological - drug effects; Thioredoxin; Tobacco; Tolerances; Transcription factors; Transgenic plants; Triticeae; Triticum - enzymology; Triticum - genetics; Triticum aestivum; Wheat
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0073989

Oxidative stress caused by accumulation of reactive oxygen species (ROS) is capable of damaging effects on numerous cellular components. Glutathione peroxidases (GPXs, EC 1.11.1.9) are key enzymes of the antioxidant network in plants. In this study, W69 and W106, two putative GPX genes, were obtained by de novo transcriptome sequencing of salt-treated wheat (Triticum aestivum) seedlings. The purified His-tag fusion proteins of W69 and W106 reduced H2O2 and t-butyl hydroperoxide (t-BHP) using glutathione (GSH) or thioredoxin (Trx) as an electron donor in vitro, showing their peroxidase activity toward H2O2 and toxic organic hydroperoxide. GFP fluorescence assays revealed that W69 and W106 are localized in chloroplasts. Quantitative real-time PCR (Q-RT-PCR) analysis showed that two GPXs were differentially responsive to salt, drought, H2O2, or ABA. Isolation of the W69 and W106 promoters revealed some cis-acting elements responding to abiotic stresses. Overexpression of W69 and W106 conferred strong tolerance to salt, H2O2, and ABA treatment in Arabidopsis. Moreover, the expression levels of key regulator genes (SOS1, RbohD and ABI1/ABI2) involved in salt, H2O2 and ABA signaling were altered in the transgenic plants. These findings suggest that W69 and W106 not only act as scavengers of H2O2 in controlling abiotic stress responses, but also play important roles in salt and ABA signaling.