Aberrant neural stem cell proliferation and increased adult neurogenesis in mice lacking chromatin protein HMGB2

Neural stem and progenitor cells (NSCs/NPCs) are distinct groups of cells found in the mammalian central nervous system (CNS). Previously we determined that members of the High Mobility Group (HMG) B family of chromatin structural proteins modulate NSC proliferation and self-renewal. Among them HMGB...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2013-12, Vol.8 (12), p.e84838-e84838
Main Authors: Abraham, Ariel B, Bronstein, Robert, Reddy, Avanish S, Maletic-Savatic, Mirjana, Aguirre, Adan, Tsirka, Stella E
Format: Article
Language:English
Subjects:
Aberration
Animals
Antigens
Biology
Blotting, Western
Brain
Cell adhesion
Cell adhesion molecules
Cell Proliferation
Cell self-renewal
Cells (biology)
Cells, Cultured
Central nervous system
Chromatin
Chromosomal proteins
Degeneration
DNA Primers - genetics
Gene regulation
High mobility group proteins
HMGB2 Protein - deficiency
HMGB2 Protein - metabolism
Kinases
Lateral Ventricles - cytology
Mice
Mice, Inbred C57BL
Mice, Knockout
Microscopy, Fluorescence
Neural cell adhesion molecule
Neural stem cells
Neural Stem Cells - metabolism
Neural Stem Cells - physiology
Neurogenesis
Neurogenesis - genetics
Neurogenesis - physiology
Oct-4 protein
Olfactory bulb
Pluripotency
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Rodents
Spermatogenesis
Statistics, Nonparametric
Stem cells
Structural proteins
Target recognition
Trauma
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Neural stem and progenitor cells (NSCs/NPCs) are distinct groups of cells found in the mammalian central nervous system (CNS). Previously we determined that members of the High Mobility Group (HMG) B family of chromatin structural proteins modulate NSC proliferation and self-renewal. Among them HMGB2 was found to be dynamically expressed in proliferating and differentiating NSCs, suggesting that it may regulate NSC maintenance. We report now that Hmgb2(-/-) mice exhibit SVZ hyperproliferation, increased numbers of SVZ NSCs, and a trend towards aberrant increases in newly born neurons in the olfactory bulb (OB) granule cell layer. Increases in the levels of the transcription factor p21 and the Neural cell adhesion molecule (NCAM), along with down-regulation of the transcription/pluripotency factor Oct4 in the Hmgb2-/- SVZ point to a possible pathway for this increased proliferation/differentiation. Our findings suggest that HMGB2 functions as a modulator of neurogenesis in young adult mice through regulation of NSC proliferation, and identify a potential target via which CNS repair could be amplified following trauma or disease-based neuronal degeneration.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0084838