Multilayered polyelectrolyte microcapsules: interaction with the enzyme cytochrome C oxidase

Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2014-11, Vol.9 (11), p.e112192-e112192
Main Authors: Pastorino, Laura, Dellacasa, Elena, Noor, Mohamed R, Soulimane, Tewfik, Bianchini, Paolo, D'Autilia, Francesca, Antipov, Alexei, Diaspro, Alberto, Tofail, Syed A M, Ruggiero, Carmelina
Format: Article
Language:English
Subjects:
Adsorption
Bacterial Proteins - chemistry
Bioengineering
Biology and Life Sciences
Capsules
Cytochrome
Cytochrome c
Cytochrome oxidase
Cytochrome-c oxidase
Drug delivery systems
Electron Transport Complex IV - chemistry
Engineering and Technology
Environmental science
Enzymes
Enzymes, Immobilized - chemistry
Fabrication
Fourier transforms
Informatics
Magnetic fields
Medicine and Health Sciences
Microcapsules
Microscopy
Oxidase
Permeability
Polyelectrolytes
Proteins
Respiration
Robotics
Studies
Thermus thermophilus
Thermus thermophilus - enzymology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0112192