Conserved nutrient sensor O-GlcNAc transferase is integral to C. elegans pathogen-specific immunity

Discriminating pathogenic bacteria from bacteria used as a food source is key to Caenorhabidits elegans immunity. Using mutants defective in the enzymes of O-linked N-acetylglucosamine (O-GlcNAc) cycling, we examined the role of this nutrient-sensing pathway in the C. elegans innate immune response....

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Bibliographic Details
Published in:PloS one 2014-12, Vol.9 (12), p.e113231-e113231
Main Authors: Bond, Michelle R, Ghosh, Salil K, Wang, Peng, Hanover, John A
Format: Article
Language:English
Subjects:
Acetylglucosamine
Analysis
Animals
Bacteria
Bacterial infections
beta Catenin - genetics
beta Catenin - metabolism
Biology and Life Sciences
Caenorhabditis elegans
Caenorhabditis elegans - genetics
Caenorhabditis elegans - immunology
Caenorhabditis elegans - microbiology
Cycles
Deregulation
Diabetes
Enzymes
Food
Food sources
Gene expression
Genetic analysis
Genomes
Humans
Immune response
Immune system
Immunity
Immunity, Innate - genetics
Innate immunity
Kidney diseases
Laboratories
Mammals
MAP kinase
Mutants
N-Acetylglucosamine
N-Acetylglucosaminyltransferases - genetics
N-Acetylglucosaminyltransferases - metabolism
Nematodes
Nutrient availability
Nutrient cycles
Nutrients
p38 Mitogen-Activated Protein Kinases - genetics
p38 Mitogen-Activated Protein Kinases - metabolism
Pathogenesis
Pathogens
Proteins
Pseudomonas aeruginosa
Research and Analysis Methods
Signal Transduction - genetics
Staphylococcus aureus - immunology
Staphylococcus aureus - pathogenicity
Transcription
Worms
β-Catenin
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Summary:Discriminating pathogenic bacteria from bacteria used as a food source is key to Caenorhabidits elegans immunity. Using mutants defective in the enzymes of O-linked N-acetylglucosamine (O-GlcNAc) cycling, we examined the role of this nutrient-sensing pathway in the C. elegans innate immune response. Genetic analysis showed that deletion of O-GlcNAc transferase (ogt-1) yielded animals hypersensitive to the human pathogen S. aureus but not to P. aeruginosa. Genetic interaction studies revealed that nutrient-responsive OGT-1 acts through the conserved β-catenin (BAR-1) pathway and in concert with p38 MAPK (PMK-1) to modulate the immune response to S. aureus. Moreover, whole genome transcriptional profiling revealed that O-GlcNAc cycling mutants exhibited deregulation of unique stress- and immune-responsive genes. The participation of nutrient sensor OGT-1 in an immunity module evolutionarily conserved from C. elegans to humans reveals an unexplored nexus between nutrient availability and a pathogen-specific immune response.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0113231