Loading…

Positive effects of bFGF modified rat amniotic epithelial cells transplantation on transected rat optic nerve

Effective therapy for visual loss caused by optic nerve injury or diseases has not been achieved even though the optic nerve has the regeneration potential after injury. This study was designed to modify amniotic epithelial cells (AECs) with basic fibroblast growth factor (bFGF) gene, preliminarily...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-03, Vol.10 (3), p.e0119119
Main Authors: Xie, Jia-Xin, Feng, Yu, Yuan, Jian-Min, You, Zhen-Dong, Lin, Hai-Yan, Lu, Chang-Lin, Xu, Jia-Jun
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c758t-8b64ff340d8d09126b0fa2d88c84bf8d9603754cb8f7ba942fbde74790b7fcca3
cites cdi_FETCH-LOGICAL-c758t-8b64ff340d8d09126b0fa2d88c84bf8d9603754cb8f7ba942fbde74790b7fcca3
container_end_page
container_issue 3
container_start_page e0119119
container_title PloS one
container_volume 10
creator Xie, Jia-Xin
Feng, Yu
Yuan, Jian-Min
You, Zhen-Dong
Lin, Hai-Yan
Lu, Chang-Lin
Xu, Jia-Jun
description Effective therapy for visual loss caused by optic nerve injury or diseases has not been achieved even though the optic nerve has the regeneration potential after injury. This study was designed to modify amniotic epithelial cells (AECs) with basic fibroblast growth factor (bFGF) gene, preliminarily investigating its effect on transected optic nerve. A human bFGF gene segment was delivered into rat AECs (AECs/hbFGF) by lentiviral vector, and the gene expression was examined by RT-PCR and ELISA. The AECs/hbFGF and untransfected rat AECs were transplanted into the transected site of the rat optic nerve. At 28 days post transplantation, the survival and migration of the transplanted cells was observed by tracking labeled cells; meanwhile retinal ganglion cells (RGCs) were observed and counted by employing biotin dextran amine (BDA) and Nissl staining. Furthermore, the expression of growth associated protein 43 (GAP-43) within the injury site was examined with immunohistochemical staining. The AECs/hbFGF was proven to express bFGF gene and secrete bFGF peptide. Both AECs/hbFGF and AECs could survive and migrate after transplantation. RGCs counting implicated that RGCs numbers of the cell transplantation groups were significantly higher than that of the control group, and the AECs/hbFGF group was significantly higher than that of the AECs group. Moreover GAP-43 integral optical density value in the control group was significantly lower than that of the cell transplantation groups, and the value in the AECs/hbFGF group was significantly higher than that of the AECs group. AECs modified with bFGF could reduce RGCs loss and promote expression of GAP-43 in the rat optic nerve transected model, facilitating the process of neural restoration following injury.
doi_str_mv 10.1371/journal.pone.0119119
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1660378325</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A425976513</galeid><doaj_id>oai_doaj_org_article_0ec14d61500d4a1fb756be8325ddd8fe</doaj_id><sourcerecordid>A425976513</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-8b64ff340d8d09126b0fa2d88c84bf8d9603754cb8f7ba942fbde74790b7fcca3</originalsourceid><addsrcrecordid>eNqNk11r2zAUhs3YWLtu_2BshsHYLpJJlmzZN4NSli5Q6NjXrZClo0RBtjxLDuu_r9y4JR69GBZYHD3vq6MjnSR5jdESE4Y_7dzQt8IuO9fCEmFcxfEkOcUVyRZFhsjTo_lJ8sL7HUI5KYvieXKS5YxQWrHTpPnmvAlmDyloDTL41Om0Xl2u0sYpow2otBchFU1rXDAyhc6ELVgjbCrBWp-GXrS-s6INIhjXpnHchaLXpHXdKGyh38PL5JkW1sOr6X-W_Fp9-XnxdXF1fbm-OL9aSJaXYVHWBdWaUKRKhSqcFTXSIlNlKUta61JVBSIsp7IuNatFRTNdK2CUVahmWkpBzpK3B9_OOs-nSnmOi1FYkiyPxPpAKCd2vOtNI_ob7oThdwHXb7joY94WOAKJqSpwjpCiAuua5UUNo4tSqtQQvT5Puw11A0pCGytgZ6bzldZs-cbtOSUxZ8aiwYfJoHd_BvCBN8aP5RUtuOGQd5WxjFQRffcP-vjpJmoj4gFMq13cV46m_JxmecWKHJNILR-h4qegMTI-K21ifCb4OBNEJsDfsBGD93z94_v_s9e_5-z7I3YLwoatd3YYH5Sfg_QAyt5534N-KDJGfOyK-2rwsSv41BVR9ub4gh5E921AbgFaCAk-</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1660378325</pqid></control><display><type>article</type><title>Positive effects of bFGF modified rat amniotic epithelial cells transplantation on transected rat optic nerve</title><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><source>PubMed Central Free</source><creator>Xie, Jia-Xin ; Feng, Yu ; Yuan, Jian-Min ; You, Zhen-Dong ; Lin, Hai-Yan ; Lu, Chang-Lin ; Xu, Jia-Jun</creator><contributor>Matsui, Jonathan I.</contributor><creatorcontrib>Xie, Jia-Xin ; Feng, Yu ; Yuan, Jian-Min ; You, Zhen-Dong ; Lin, Hai-Yan ; Lu, Chang-Lin ; Xu, Jia-Jun ; Matsui, Jonathan I.</creatorcontrib><description>Effective therapy for visual loss caused by optic nerve injury or diseases has not been achieved even though the optic nerve has the regeneration potential after injury. This study was designed to modify amniotic epithelial cells (AECs) with basic fibroblast growth factor (bFGF) gene, preliminarily investigating its effect on transected optic nerve. A human bFGF gene segment was delivered into rat AECs (AECs/hbFGF) by lentiviral vector, and the gene expression was examined by RT-PCR and ELISA. The AECs/hbFGF and untransfected rat AECs were transplanted into the transected site of the rat optic nerve. At 28 days post transplantation, the survival and migration of the transplanted cells was observed by tracking labeled cells; meanwhile retinal ganglion cells (RGCs) were observed and counted by employing biotin dextran amine (BDA) and Nissl staining. Furthermore, the expression of growth associated protein 43 (GAP-43) within the injury site was examined with immunohistochemical staining. The AECs/hbFGF was proven to express bFGF gene and secrete bFGF peptide. Both AECs/hbFGF and AECs could survive and migrate after transplantation. RGCs counting implicated that RGCs numbers of the cell transplantation groups were significantly higher than that of the control group, and the AECs/hbFGF group was significantly higher than that of the AECs group. Moreover GAP-43 integral optical density value in the control group was significantly lower than that of the cell transplantation groups, and the value in the AECs/hbFGF group was significantly higher than that of the AECs group. AECs modified with bFGF could reduce RGCs loss and promote expression of GAP-43 in the rat optic nerve transected model, facilitating the process of neural restoration following injury.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0119119</identifier><identifier>PMID: 25734497</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amnion - cytology ; Animals ; Biomarkers - metabolism ; Biotin ; Cell Count ; Cell Differentiation ; Cell migration ; Cell Survival ; Counting ; Dextran ; Dextrans ; Enzyme-linked immunosorbent assay ; Epithelial cells ; Epithelial Cells - cytology ; Epithelial Cells - metabolism ; Epithelial Cells - transplantation ; Fibroblast growth factor 2 ; Fibroblast Growth Factor 2 - genetics ; Fibroblast Growth Factor 2 - metabolism ; Fibroblast growth factors ; Fibroblasts ; GAP-43 protein ; GAP-43 Protein - genetics ; GAP-43 Protein - metabolism ; Gene Expression ; Genetic Vectors ; Growth factors ; Humans ; Injuries ; Laboratory animals ; Lentivirus - genetics ; Male ; Nerve Regeneration - physiology ; Nervous system ; Neurobiology ; Neurosciences ; Optic nerve ; Optic Nerve - metabolism ; Optic Nerve - pathology ; Optic Nerve - surgery ; Optic Nerve Injuries - metabolism ; Optic Nerve Injuries - pathology ; Optic Nerve Injuries - therapy ; Optical density ; Polymerase chain reaction ; Proteins ; Rats ; Rats, Sprague-Dawley ; Regeneration ; Restoration ; Retina ; Retinal ganglion cells ; Retinal Ganglion Cells - cytology ; Retinal Ganglion Cells - metabolism ; Rodents ; Spinal cord injuries ; Staining ; Transduction, Genetic ; Transgenes ; Transplantation ; Vectors (Biology)</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0119119</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Xie et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Xie et al 2015 Xie et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-8b64ff340d8d09126b0fa2d88c84bf8d9603754cb8f7ba942fbde74790b7fcca3</citedby><cites>FETCH-LOGICAL-c758t-8b64ff340d8d09126b0fa2d88c84bf8d9603754cb8f7ba942fbde74790b7fcca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1660378325/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1660378325?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25734497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Matsui, Jonathan I.</contributor><creatorcontrib>Xie, Jia-Xin</creatorcontrib><creatorcontrib>Feng, Yu</creatorcontrib><creatorcontrib>Yuan, Jian-Min</creatorcontrib><creatorcontrib>You, Zhen-Dong</creatorcontrib><creatorcontrib>Lin, Hai-Yan</creatorcontrib><creatorcontrib>Lu, Chang-Lin</creatorcontrib><creatorcontrib>Xu, Jia-Jun</creatorcontrib><title>Positive effects of bFGF modified rat amniotic epithelial cells transplantation on transected rat optic nerve</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Effective therapy for visual loss caused by optic nerve injury or diseases has not been achieved even though the optic nerve has the regeneration potential after injury. This study was designed to modify amniotic epithelial cells (AECs) with basic fibroblast growth factor (bFGF) gene, preliminarily investigating its effect on transected optic nerve. A human bFGF gene segment was delivered into rat AECs (AECs/hbFGF) by lentiviral vector, and the gene expression was examined by RT-PCR and ELISA. The AECs/hbFGF and untransfected rat AECs were transplanted into the transected site of the rat optic nerve. At 28 days post transplantation, the survival and migration of the transplanted cells was observed by tracking labeled cells; meanwhile retinal ganglion cells (RGCs) were observed and counted by employing biotin dextran amine (BDA) and Nissl staining. Furthermore, the expression of growth associated protein 43 (GAP-43) within the injury site was examined with immunohistochemical staining. The AECs/hbFGF was proven to express bFGF gene and secrete bFGF peptide. Both AECs/hbFGF and AECs could survive and migrate after transplantation. RGCs counting implicated that RGCs numbers of the cell transplantation groups were significantly higher than that of the control group, and the AECs/hbFGF group was significantly higher than that of the AECs group. Moreover GAP-43 integral optical density value in the control group was significantly lower than that of the cell transplantation groups, and the value in the AECs/hbFGF group was significantly higher than that of the AECs group. AECs modified with bFGF could reduce RGCs loss and promote expression of GAP-43 in the rat optic nerve transected model, facilitating the process of neural restoration following injury.</description><subject>Amnion - cytology</subject><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Biotin</subject><subject>Cell Count</subject><subject>Cell Differentiation</subject><subject>Cell migration</subject><subject>Cell Survival</subject><subject>Counting</subject><subject>Dextran</subject><subject>Dextrans</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - transplantation</subject><subject>Fibroblast growth factor 2</subject><subject>Fibroblast Growth Factor 2 - genetics</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Fibroblast growth factors</subject><subject>Fibroblasts</subject><subject>GAP-43 protein</subject><subject>GAP-43 Protein - genetics</subject><subject>GAP-43 Protein - metabolism</subject><subject>Gene Expression</subject><subject>Genetic Vectors</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Injuries</subject><subject>Laboratory animals</subject><subject>Lentivirus - genetics</subject><subject>Male</subject><subject>Nerve Regeneration - physiology</subject><subject>Nervous system</subject><subject>Neurobiology</subject><subject>Neurosciences</subject><subject>Optic nerve</subject><subject>Optic Nerve - metabolism</subject><subject>Optic Nerve - pathology</subject><subject>Optic Nerve - surgery</subject><subject>Optic Nerve Injuries - metabolism</subject><subject>Optic Nerve Injuries - pathology</subject><subject>Optic Nerve Injuries - therapy</subject><subject>Optical density</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regeneration</subject><subject>Restoration</subject><subject>Retina</subject><subject>Retinal ganglion cells</subject><subject>Retinal Ganglion Cells - cytology</subject><subject>Retinal Ganglion Cells - metabolism</subject><subject>Rodents</subject><subject>Spinal cord injuries</subject><subject>Staining</subject><subject>Transduction, Genetic</subject><subject>Transgenes</subject><subject>Transplantation</subject><subject>Vectors (Biology)</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11r2zAUhs3YWLtu_2BshsHYLpJJlmzZN4NSli5Q6NjXrZClo0RBtjxLDuu_r9y4JR69GBZYHD3vq6MjnSR5jdESE4Y_7dzQt8IuO9fCEmFcxfEkOcUVyRZFhsjTo_lJ8sL7HUI5KYvieXKS5YxQWrHTpPnmvAlmDyloDTL41Om0Xl2u0sYpow2otBchFU1rXDAyhc6ELVgjbCrBWp-GXrS-s6INIhjXpnHchaLXpHXdKGyh38PL5JkW1sOr6X-W_Fp9-XnxdXF1fbm-OL9aSJaXYVHWBdWaUKRKhSqcFTXSIlNlKUta61JVBSIsp7IuNatFRTNdK2CUVahmWkpBzpK3B9_OOs-nSnmOi1FYkiyPxPpAKCd2vOtNI_ob7oThdwHXb7joY94WOAKJqSpwjpCiAuua5UUNo4tSqtQQvT5Puw11A0pCGytgZ6bzldZs-cbtOSUxZ8aiwYfJoHd_BvCBN8aP5RUtuOGQd5WxjFQRffcP-vjpJmoj4gFMq13cV46m_JxmecWKHJNILR-h4qegMTI-K21ifCb4OBNEJsDfsBGD93z94_v_s9e_5-z7I3YLwoatd3YYH5Sfg_QAyt5534N-KDJGfOyK-2rwsSv41BVR9ub4gh5E921AbgFaCAk-</recordid><startdate>20150303</startdate><enddate>20150303</enddate><creator>Xie, Jia-Xin</creator><creator>Feng, Yu</creator><creator>Yuan, Jian-Min</creator><creator>You, Zhen-Dong</creator><creator>Lin, Hai-Yan</creator><creator>Lu, Chang-Lin</creator><creator>Xu, Jia-Jun</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150303</creationdate><title>Positive effects of bFGF modified rat amniotic epithelial cells transplantation on transected rat optic nerve</title><author>Xie, Jia-Xin ; Feng, Yu ; Yuan, Jian-Min ; You, Zhen-Dong ; Lin, Hai-Yan ; Lu, Chang-Lin ; Xu, Jia-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-8b64ff340d8d09126b0fa2d88c84bf8d9603754cb8f7ba942fbde74790b7fcca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amnion - cytology</topic><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Biotin</topic><topic>Cell Count</topic><topic>Cell Differentiation</topic><topic>Cell migration</topic><topic>Cell Survival</topic><topic>Counting</topic><topic>Dextran</topic><topic>Dextrans</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - transplantation</topic><topic>Fibroblast growth factor 2</topic><topic>Fibroblast Growth Factor 2 - genetics</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Fibroblast growth factors</topic><topic>Fibroblasts</topic><topic>GAP-43 protein</topic><topic>GAP-43 Protein - genetics</topic><topic>GAP-43 Protein - metabolism</topic><topic>Gene Expression</topic><topic>Genetic Vectors</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Injuries</topic><topic>Laboratory animals</topic><topic>Lentivirus - genetics</topic><topic>Male</topic><topic>Nerve Regeneration - physiology</topic><topic>Nervous system</topic><topic>Neurobiology</topic><topic>Neurosciences</topic><topic>Optic nerve</topic><topic>Optic Nerve - metabolism</topic><topic>Optic Nerve - pathology</topic><topic>Optic Nerve - surgery</topic><topic>Optic Nerve Injuries - metabolism</topic><topic>Optic Nerve Injuries - pathology</topic><topic>Optic Nerve Injuries - therapy</topic><topic>Optical density</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regeneration</topic><topic>Restoration</topic><topic>Retina</topic><topic>Retinal ganglion cells</topic><topic>Retinal Ganglion Cells - cytology</topic><topic>Retinal Ganglion Cells - metabolism</topic><topic>Rodents</topic><topic>Spinal cord injuries</topic><topic>Staining</topic><topic>Transduction, Genetic</topic><topic>Transgenes</topic><topic>Transplantation</topic><topic>Vectors (Biology)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Jia-Xin</creatorcontrib><creatorcontrib>Feng, Yu</creatorcontrib><creatorcontrib>Yuan, Jian-Min</creatorcontrib><creatorcontrib>You, Zhen-Dong</creatorcontrib><creatorcontrib>Lin, Hai-Yan</creatorcontrib><creatorcontrib>Lu, Chang-Lin</creatorcontrib><creatorcontrib>Xu, Jia-Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale_Opposing Viewpoints In Context</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection (ProQuest Medical &amp; Health Databases)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Jia-Xin</au><au>Feng, Yu</au><au>Yuan, Jian-Min</au><au>You, Zhen-Dong</au><au>Lin, Hai-Yan</au><au>Lu, Chang-Lin</au><au>Xu, Jia-Jun</au><au>Matsui, Jonathan I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Positive effects of bFGF modified rat amniotic epithelial cells transplantation on transected rat optic nerve</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-03-03</date><risdate>2015</risdate><volume>10</volume><issue>3</issue><spage>e0119119</spage><pages>e0119119-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Effective therapy for visual loss caused by optic nerve injury or diseases has not been achieved even though the optic nerve has the regeneration potential after injury. This study was designed to modify amniotic epithelial cells (AECs) with basic fibroblast growth factor (bFGF) gene, preliminarily investigating its effect on transected optic nerve. A human bFGF gene segment was delivered into rat AECs (AECs/hbFGF) by lentiviral vector, and the gene expression was examined by RT-PCR and ELISA. The AECs/hbFGF and untransfected rat AECs were transplanted into the transected site of the rat optic nerve. At 28 days post transplantation, the survival and migration of the transplanted cells was observed by tracking labeled cells; meanwhile retinal ganglion cells (RGCs) were observed and counted by employing biotin dextran amine (BDA) and Nissl staining. Furthermore, the expression of growth associated protein 43 (GAP-43) within the injury site was examined with immunohistochemical staining. The AECs/hbFGF was proven to express bFGF gene and secrete bFGF peptide. Both AECs/hbFGF and AECs could survive and migrate after transplantation. RGCs counting implicated that RGCs numbers of the cell transplantation groups were significantly higher than that of the control group, and the AECs/hbFGF group was significantly higher than that of the AECs group. Moreover GAP-43 integral optical density value in the control group was significantly lower than that of the cell transplantation groups, and the value in the AECs/hbFGF group was significantly higher than that of the AECs group. AECs modified with bFGF could reduce RGCs loss and promote expression of GAP-43 in the rat optic nerve transected model, facilitating the process of neural restoration following injury.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25734497</pmid><doi>10.1371/journal.pone.0119119</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2015-03, Vol.10 (3), p.e0119119
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1660378325
source Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central Free
subjects Amnion - cytology
Animals
Biomarkers - metabolism
Biotin
Cell Count
Cell Differentiation
Cell migration
Cell Survival
Counting
Dextran
Dextrans
Enzyme-linked immunosorbent assay
Epithelial cells
Epithelial Cells - cytology
Epithelial Cells - metabolism
Epithelial Cells - transplantation
Fibroblast growth factor 2
Fibroblast Growth Factor 2 - genetics
Fibroblast Growth Factor 2 - metabolism
Fibroblast growth factors
Fibroblasts
GAP-43 protein
GAP-43 Protein - genetics
GAP-43 Protein - metabolism
Gene Expression
Genetic Vectors
Growth factors
Humans
Injuries
Laboratory animals
Lentivirus - genetics
Male
Nerve Regeneration - physiology
Nervous system
Neurobiology
Neurosciences
Optic nerve
Optic Nerve - metabolism
Optic Nerve - pathology
Optic Nerve - surgery
Optic Nerve Injuries - metabolism
Optic Nerve Injuries - pathology
Optic Nerve Injuries - therapy
Optical density
Polymerase chain reaction
Proteins
Rats
Rats, Sprague-Dawley
Regeneration
Restoration
Retina
Retinal ganglion cells
Retinal Ganglion Cells - cytology
Retinal Ganglion Cells - metabolism
Rodents
Spinal cord injuries
Staining
Transduction, Genetic
Transgenes
Transplantation
Vectors (Biology)
title Positive effects of bFGF modified rat amniotic epithelial cells transplantation on transected rat optic nerve
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T13%3A07%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Positive%20effects%20of%20bFGF%20modified%20rat%20amniotic%20epithelial%20cells%20transplantation%20on%20transected%20rat%20optic%20nerve&rft.jtitle=PloS%20one&rft.au=Xie,%20Jia-Xin&rft.date=2015-03-03&rft.volume=10&rft.issue=3&rft.spage=e0119119&rft.pages=e0119119-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0119119&rft_dat=%3Cgale_plos_%3EA425976513%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c758t-8b64ff340d8d09126b0fa2d88c84bf8d9603754cb8f7ba942fbde74790b7fcca3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1660378325&rft_id=info:pmid/25734497&rft_galeid=A425976513&rfr_iscdi=true