Abnormal development of tapetum and microspores induced by chemical hybridization agent SQ-1 in wheat

Chemical hybridization agent (CHA)-induced male sterility is an important tool in crop heterosis. To demonstrate that CHA-SQ-1-induced male sterility is associated with abnormal tapetal and microspore development, the cytology of CHA-SQ-1-treated plant anthers at various developmental stages was stu...

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Bibliographic Details
Published in:PloS one 2015-03, Vol.10 (3), p.e0119557-e0119557
Main Authors: Wang, Shuping, Zhang, Gaisheng, Song, Qilu, Zhang, Yingxin, Li, Zheng, Guo, Jialin, Niu, Na, Ma, Shoucai, Wang, Junwei
Format: Article
Language:English
Subjects:
Abortion
Agricultural biotechnology
Agronomy
Anthers
Apoptosis
Apoptosis - drug effects
Cell death
Cytology
Developmental stages
Education
Electron microscopy
Engineering research
Fertility
Flowers - cytology
Flowers - drug effects
Fluorescein
Fluorescein diacetate
Gametogenesis, Plant - drug effects
Gametogenesis, Plant - genetics
Gene expression
Heterosis
Hybrid Vigor
Hybridization
In Situ Nick-End Labeling
Labeling
Labelling
Laboratories
Light microscopy
Male sterility
Microscopy
Microspores
Molecular biology
Plant Infertility - drug effects
Plant reproduction
Pollen
Pollen - cytology
Pollen - drug effects
Pyridazines - pharmacology
Rice
Scanning electron microscopy
Seeds
Staining
Sterility
Tobacco
Transcription factors
Transmission electron microscopy
Triticum - drug effects
Triticum - genetics
Triticum - growth & development
Viability
Wheat
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Summary:Chemical hybridization agent (CHA)-induced male sterility is an important tool in crop heterosis. To demonstrate that CHA-SQ-1-induced male sterility is associated with abnormal tapetal and microspore development, the cytology of CHA-SQ-1-treated plant anthers at various developmental stages was studied by light microscopy, scanning and transmission electron microscopy, in situ terminal deoxynucleotidyl transferasemediated dUTP nick end-labelling (TUNEL) assay and DAPI staining. The results indicated that the SQ-1-treated plants underwent premature tapetal programmed cell death (PCD), which was initiated at the early-uninucleate stage of microspore development and continued until the tapetal cells were completely degraded; the process of microspore development was then blocked. Microspores with low-viability (fluorescein diacetate staining) were aborted. The study suggests that premature tapetal PCD is the main cause of pollen abortion. Furthermore, it determines the starting period and a key factor in CHA-SQ-1-induced male sterility at the cell level, and provides cytological evidence to further study the mechanism between PCD and male sterility.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0119557