Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination

Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understand...

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Published in:PloS one 2015-07, Vol.10 (7), p.e0133444-e0133444
Main Authors: Wu, You, Chen, Ping, Jing, Yuanya, Wang, Chen, Men, Yu-Long, Zhan, Wang, Wang, Qiang, Gan, Zhixue, Huang, Jin, Xie, Kun, Mi, Jiangsheng, Yu, Chenghua, Yu, Xiuqing, Chen, Pei-Chao, Chang, Jian-Feng, Cai, Fengfeng, Chen, Su
Format: Article
Language:English
Subjects:
Animals
Bioinformatics
Biological activity
Biological effects
Breast cancer
Cell cycle
Cell Differentiation - genetics
Chromatin
Chromatin - metabolism
Data processing
Deoxyribonucleic acid
DNA
DNA Damage - genetics
DNA methylation
DNA microarrays
DNA Repair - genetics
Education
Gene Expression Profiling
Gene Expression Regulation
Gene Knockdown Techniques
Genes
Genomes
HEK293 Cells
HeLa Cells
Histone H2B
Histones - genetics
Histones - metabolism
Humans
Life sciences
Mammals
Medicine
Mice
Mouse Embryonic Stem Cells - cytology
Mutant Proteins - metabolism
Mutation - genetics
Oligonucleotide Array Sequence Analysis
Stem cells
Surgery
Transcription
Tumorigenesis
Ubiquitin - metabolism
Ubiquitination
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Summary:Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understanding of biological roles of H2B monoubiquitination is still poorly understood. In the present study, we developed an efficient tool to disrupt endogenous H2B monoubiquitination levels by using an H2BK120R mutant construct expressed in human cells. Genome-wide microarray analysis of these cells revealed a potential global view of biological functions of H2B monoubiquitination. Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes. Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0133444