Microplasma Induced Cell Morphological Changes and Apoptosis of Ex Vivo Cultured Human Anterior Lens Epithelial Cells - Relevance to Capsular Opacification

• Published in: PloS one 2016-11, Vol.11 (11), p.e0165883-e0165883
• Main Authors: Recek, Nina, Andjelić, Sofija, Hojnik, Nataša, Filipič, Gregor, Lazović, Saša, Vesel, Alenka, Primc, Gregor, Mozetič, Miran, Hawlina, Marko, Petrovski, Goran, Cvelbar, Uroš
• Format: Article
• Language: English
• Subjects: Apoptosis; Atmospheric pressure; Biology; Biology and Life Sciences; Capsule Opacification - therapy; Caspase; Caspase-8; Cataracts; Cells, Cultured; Cellular structure; Charged particles; CXCR4 protein; Deoxyribonucleic acid; Discharge; DNA; DNA fragmentation; Electrodes; Engineering; Epithelial cells; Epithelial Cells - cytology; Equipment Design; Eye surgery; Free radicals; Gas flow; Hospitals; Humans; Lens Capsule, Crystalline - cytology; Lenses; Lipid peroxidation; Lipids; Medicine and Health Sciences; Micromanipulation - instrumentation; Microscopy; Morphology; Ophthalmology; Physical Sciences; Physics; Plasma; Plasma Gases - administration & dosage; Plasma Gases - therapeutic use; Stem cells; Surgery
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0165883

Inducing selective or targeted cell apoptosis without affecting large number of neighbouring cells remains a challenge. A plausible method for treatment of posterior capsular opacification (PCO) due to remaining lens epithelial cells (LECs) by reactive chemistry induced by localized single electrode microplasma discharge at top of a needle-like glass electrode with spot size ~3 μm is hereby presented. The focused and highly-localized atmospheric pressure microplasma jet with electrode discharge could induce a dose-dependent apoptosis in selected and targeted individual LECs, which could be confirmed by real-time monitoring of the morphological and structural changes at cellular level. Direct cell treatment with microplasma inside the medium appeared more effective in inducing apoptosis (caspase 8 positivity and DNA fragmentation) at a highly targeted cell level compared to treatment on top of the medium (indirect treatment). Our results show that single cell specific micropipette plasma can be used to selectively induce demise in LECs which remain in the capsular bag after cataract surgery and thus prevent their migration (CXCR4 positivity) to the posterior lens capsule and PCO formation.