Evaluation of the Presence and Levels of Enrofloxacin, Ciprofloxacin, Sulfaquinoxaline and Oxytetracycline in Broiler Chickens after Drug Administration

• Published in: PloS one 2016-11, Vol.11 (11), p.e0166402-e0166402
• Main Authors: de Assis, Débora Cristina Sampaio, da Silva, Guilherme Resende, Lanza, Isabela Pereira, Ribeiro, Ana Cláudia Dos Santos Rossi, Lana, Ângela Maria Quintão, Lara, Leonardo José Camargos, de Figueiredo, Tadeu Chaves, Cançado, Silvana de Vasconcelos
• Format: Article
• Language: English
• Subjects: Analysis; Analytical chemistry; Animal Diseases - drug therapy; Animal Diseases - microbiology; Animals; Anti-infective agents; Anti-Infective Agents - administration & dosage; Anti-Infective Agents - isolation & purification; Antibiotics; Antimicrobial agents; Biology and Life Sciences; Chickens; Chickens - microbiology; Chromatography; Ciprofloxacin; Ciprofloxacin - administration & dosage; Ciprofloxacin - isolation & purification; Drug Residues - chemistry; Drug Residues - isolation & purification; Drug therapy; Drugs; Enrofloxacin; Fluoroquinolones - administration & dosage; Fluoroquinolones - isolation & purification; Food; Gram-positive bacteria; Inactivation, Metabolic; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Medicine and Health Sciences; Muscles; Muscles - chemistry; Muscles - drug effects; Oxytetracycline; Oxytetracycline - administration & dosage; Oxytetracycline - isolation & purification; Pharmacology; Poultry; Residues; Scientific imaging; Studies; Sulfaquinoxaline - administration & dosage; Sulfaquinoxaline - isolation & purification
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0166402

The depletion times of enrofloxacin and its metabolite ciprofloxacin as well as sulfaquinoxaline and oxytetracycline were evaluated in broiler chickens that had been subjected to pharmacological treatment. The presence and residue levels of these drugs in muscle tissue were evaluated using an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method that was validated in this work. The results showed the presence of all antimicrobial residues; however, the presence of residues at concentrations higher than the drugs' maximum residue limit (MRL) of 100 μg kg-1 was found only during the treatment period for oxytetracycline and until two days after discontinuation of the medication for enrofloxacin, ciprofloxacin and sulfaquinoxaline. It was concluded that the residues of all antimicrobials were rapidly metabolized from the broiler muscles; after four days of withdrawal, the levels were lower than the limit of quantification (LOQ) of the method for the studied analytes.