Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging

The Ames dwarf (df/df) mice have extended longevity and can preserve the ovarian reserve longer than Normal (N) mice. Based on this, the aim of our study was to evaluate the ovarian microRNA (miRNA) profile in young and aged df/df and N mice. Ovarian tissue was collected at 5-6 months and at 21-22 m...

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Bibliographic Details
Published in:PloS one 2017-01, Vol.12 (1), p.e0169213-e0169213
Main Authors: Schneider, Augusto, Matkovich, Scot J, Victoria, Berta, Spinel, Lina, Bartke, Andrzej, Golusinski, Pawel, Masternak, Michal M
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
Age
Aging
Aging (Biology)
Aging - genetics
AKT protein
Animals
Biology
Biology and life sciences
Breast cancer
Cell adhesion & migration
Cluster Analysis
Female
Forkhead protein
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Ontology
Genetic aspects
Genotypes
Health aspects
House mouse
Insulin
Longevity - genetics
Medicine
Medicine and Health Sciences
Mice
MicroRNA
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Ovarian cancer
Ovaries
Ovary - metabolism
Puberty
Research and Analysis Methods
Ribonucleic acid
RNA
Rodents
Signaling
Stem cells
TOR protein
Transgenic animals
Womens health
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Summary:The Ames dwarf (df/df) mice have extended longevity and can preserve the ovarian reserve longer than Normal (N) mice. Based on this, the aim of our study was to evaluate the ovarian microRNA (miRNA) profile in young and aged df/df and N mice. Ovarian tissue was collected at 5-6 months and at 21-22 months of age for miRNA sequencing. We detected a total of 404 miRNAs in the ovarian samples, from which the abundance of 22 and 33 miRNAs changed with age in N and df/df mice, respectively. Of these, only three miRNAs were commonly regulated with age between N and df/df mice, indicating a very divergent miRNA profile between genotypes. We also detected that 46 miRNAs were regulated between N and df/df mice, of which 23 were regulated exclusively in young mice, 12 exclusively in old mice and 12 commonly regulated at young and old ages. Many genes likely to be targeted by these miRNAs are involved in the FoxO, mTOR, PI3k/Akt and insulin signaling pathways. These results suggest that the aging process has a differential impact on the ovarian miRNA profile in df/df mice, and suggest that these miRNAs can be central players in the maintenance of a younger ovarian phenotype.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0169213