The role of integration in oncogenic progression of HPV-associated cancers

• Published in: PLoS pathogens 2017-04, Vol.13 (4), p.e1006211-e1006211
• Main Authors: McBride, Alison A, Warburton, Alix
• Format: Article
• Language: English
• Subjects: Abrasion; Abrasion resistance; Allergies; Amplification; Anogenital; Asymmetry; Basal cells; Binding sites; Biology and life sciences; c-Myc protein; Cancer; Carcinogenesis; Carcinogens; Care and treatment; Cell cycle; Cervical cancer; Cervical carcinoma; Chromatin; Chromosome rearrangements; Chromosomes; Copy number; Data acquisition; Data collection; Data processing; Deoxyribonucleic acid; Development and progression; Differentiation; DNA; DNA methylation; Epithelium; Fragile sites; Gene expression; Gene silencing; Genomes; Genomic instability; Head; Health risks; Human papillomavirus; Human pathology; Humans; Hypersensitivity; Inactivation; Infections; Infectious diseases; Laboratories; Lesions; Life cycles; Medical prognosis; Medicine and Health Sciences; Multiplexing; Neoplasms - virology; Papillomaviridae - genetics; Papillomavirus infections; Papillomavirus Infections - virology; Pearls; Probability theory; Proteins; Ribonucleic acid; RNA; Tumors; Viral diseases; Virions; Virus Integration - physiology; Viruses
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1006211

About the Authors: Alison A. McBride * E-mail: [e-mail] Affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America ORCID http://orcid.org/0000-0001-5607-5157 Alix Warburton Affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of AmericaCitation: McBride AA, Warburton A (2017) The role of integration in oncogenic progression of HPV-associated cancers. [...]integration is a dead end for the virus, as it is no longer able to form a small, circular genome that can be packaged and transmitted to a new host. [...]HPV-associated cancers are dependent on the expression of the viral E6 and E7 oncogenes for continued proliferation and survival [7]. HPV integration can be classified into two types: in Type 1, a single genome is integrated into cellular DNA; and in Type 2, multiple tandem head-to-tail repeats of the genome, in some cases with intervening cellular flanking sequences, are found at a single genomic locus [6] (see Fig 1). Continued expression of the E1 and E2 replication proteins from integrated genomes causes focal genomic instability at the integration locus [10, 11], and in most cases, when complete genomes are tandemly integrated, the internal copies are silenced by DNA methylation [12]. Common Fragile Sites and Extremely Large are Targets for Human Papillomavirus Integrations and Chromosome Rearrangements in Oropharyngeal Squamous Cell Carcinoma. Comprehensive analysis of HPV16 integration in OSCC reveals no significant impact of physical status on viral oncogene and...