Association of cord blood chemokines and other biomarkers with neonatal complications following intrauterine inflammation

• Published in: PloS one 2017-05, Vol.12 (5), p.e0175082-e0175082
• Main Authors: Otsubo, Yoshikazu, Hashimoto, Kunio, Kanbe, Taro, Sumi, Muneichiro, Moriuchi, Hiroyuki
• Format: Article
• Language: English
• Subjects: Adult; Age; Biological markers; Biology and Life Sciences; Biomarkers; Biomarkers - blood; Birth; Blood; Brain; Brain natriuretic peptide; Chemokines; Chemokines - blood; Childbirth & labor; Complications; Complications and side effects; Cord blood; Cytokines; Diagnostic systems; Female; Fetal Blood - metabolism; Fetuses; Granulocytes; Growth factors; Health aspects; Humans; Immunoassay; Infant, Newborn; Infant, Newborn, Diseases - immunology; Infant, Premature; Inflammation; Inflammation - metabolism; Intensive Care Units, Neonatal; Intercellular Signaling Peptides and Proteins - blood; Interleukin 8; Intrauterine blood transfusion; Lung diseases; Lymphocytes T; Maternal Age; Medical records; Medicine and Health Sciences; Monocyte chemoattractant protein 1; Neonates; Newborn babies; Patients; Pediatrics; Pregnancy; Premature birth; Proteins; Respiratory distress syndrome; Retrospective Studies; Risk factors; Sepsis; Th1 Cells - immunology; Th2 Cells - immunology; Tumor necrosis factor-TNF; University graduates; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0175082

Intrauterine inflammation has been associated with preterm birth and neonatal complications. Few reports have comprehensively investigated multiple cytokine profiles in cord blood and precisely identified surrogate markers for intrauterine inflammation. To identify the cytokines and surrogate markers associated with intrauterine inflammation and subsequent neonatal complications. We analyzed cord blood samples from 135 patients admitted to the neonatal intensive care unit at Sasebo City General Hospital. We retrospectively determined the associations between the presence of neonatal complications and cord blood cytokines, prenatal factors, and laboratory data at birth. A total of 27 cytokines in the cord blood were measured using a bead-based array sandwich immunoassay. Both Th1 and Th2 cytokine levels were low, whereas the levels of growth factors and chemokines were high. In particular, chemokines IL-8, MCP-1, and MIP-1α were significantly higher in very premature neonates when compared with more mature neonates. In addition, some have been shown to be associated with multiple neonatal complications, including patent ductus arteriosus (PDA), respiratory distress syndrome (RDS), and chronic lung disease (CLD). Similarly, the levels of N-terminal pro-brain natriuretic peptide, nucleated RBC, and urinary β2-microglobulin were associated with these complications and chemokine levels. Our results suggest the association of inflammatory chemokines IL-8, MCP-1, and MIP-1α with intrauterine inflammation, premature birth, and neonatal complications in these perinatal subjects. Furthermore, the association of the aforementioned biomarkers with PDA, RDS, and CLD may help establish early diagnostic measures to predict such neonatal complications following intrauterine inflammation.