Optic tract injury after closed head traumatic brain injury in mice: A model of indirect traumatic optic neuropathy

• Published in: PloS one 2018-05, Vol.13 (5), p.e0197346-e0197346
• Main Authors: Evanson, Nathan K, Guilhaume-Correa, Fernanda, Herman, James P, Goodman, Michael D
• Format: Article
• Language: English
• Subjects: Animal cognition; Biology and Life Sciences; Blue stain; Brain; Brain damage; Brain injuries; Care and treatment; Change detection; Colliculus; Complications and side effects; Computed tomography; Cytokines; Damage detection; Degeneration; Development and progression; Eye injuries; Glial fibrillary acidic protein; Gliosis; Head; Head injuries; Immunohistochemistry; Inflammation; Lateral geniculate nucleus; Magnetic resonance; Magnetic resonance imaging; Medical imaging; Medicine and Health Sciences; Methods; Mice; Microglia; Myelin; Neurodegeneration; Neuroimaging; Neurology; Neuropathy; Nuclei; Optic neuropathy; Optic tract; Pathology; Pediatrics; Research and Analysis Methods; Rodents; Skull; Staining; Stains; Stains & staining; Substantia alba; Sucrose; Superior colliculus; Suprachiasmatic nucleus; Thalamus; Traumatic brain injury
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0197346

Adult male C57BL/6J mice have previously been reported to have motor and memory deficits after experimental closed head traumatic brain injury (TBI), without associated gross pathologic damage or neuroimaging changes detectable by magnetic resonance imaging or diffusion tensor imaging protocols. The presence of neurologic deficits, however, suggests neural damage or dysfunction in these animals. Accordingly, we undertook a histologic analysis of mice after TBI. Gross pathology and histologic analysis using Nissl stain and NeuN immunohistochemistry demonstrated no obvious tissue damage or neuron loss. However, Luxol Fast Blue stain revealed myelin injury in the optic tract, while Fluoro Jade B and silver degeneration staining revealed evidence of axonal neurodegeneration in the optic tract as well as the lateral geniculate nucleus of the thalamus and superior colliculus (detectable at 7 days, but not 24 hours, after injury). Fluoro Jade B staining was not detectable in other white matter tracts, brain regions or in cell somata. In addition, there was increased GFAP staining in these optic tract, lateral geniculate, and superior colliculus 7 days post-injury, and morphologic changes in optic tract microglia that were detectable 24 hours after injury but were more prominent 7 days post-injury. Interestingly, there were no findings of degeneration or gliosis in the suprachiasmatic nucleus, which is also heavily innervated by the optic tract. Using micro-computed tomography imaging, we also found that the optic canal appears to decrease in diameter with a dorsal-ventral load on the skull, which suggests that the optic canal may be the site of injury. These results suggest that there is axonal degeneration in the optic tract and a subset of directly innervated areas, with associated neuroinflammation and astrocytosis, which develop within 7 days of injury, and also suggest that this weight drop injury may be a model for studying indirect traumatic optic neuropathy.