Virus load and clinical features during the acute phase of Chikungunya infection in children

• Published in: PloS one 2019-02, Vol.14 (2), p.e0211036-e0211036
• Main Authors: B, Siva Raghavendhar, Patel, Ashok Kumar, Kabra, Sushil Kumar, Lodha, Rakesh, Ratageri, Vinod H, Ray, Pratima
• Format: Article
• Language: English
• Subjects: Acute Disease; Adolescent; Analysis; Aquatic insects; Arthralgia; Biology and life sciences; Care and treatment; Chikungunya fever; Chikungunya Fever - blood; Chikungunya virus; Child; Child, Preschool; Children; Copy number; Cross-Sectional Studies; Cytokines; Deoxyribonucleic acid; DNA; Female; Fever; Headache; Humans; Infant; Infection; Infections; Male; Medicine and Health Sciences; Morbidity; Mosquitoes; Myalgia; Pain; Pathogenesis; Patients; Pediatric research; Pediatrics; Polymerase chain reaction; Rash; Real-Time Polymerase Chain Reaction; Research and analysis methods; Reverse Transcriptase Polymerase Chain Reaction; Reverse transcription; RNA, Viral - blood; Statistical analysis; Vector-borne diseases; Viral Load; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0211036

Chikungunya virus (CHIKV) infection is a long known mosquito-borne disease that is associated with severe morbidity, characterized by fever, headache, rashes, joint pain, and myalgia. It is believed that virus load has relation with severity of clinical features. We performed this study to assess the relationship between virus load and clinical features in children during the acute phase of CHIKV infection, in order to draw insights for better-informed treatment. Between June 1, 2009, and May 31, 2010, 338 patients with fever and susceptive to CHIKV during first 4 days of illness were prospectively enrolled from Karnataka Institute of Medical Sciences, Hubli in our hospital based cross sectional observational study. Sybr green quantitative reverse transcription polymerase chain reaction was performed to estimate the virus load. Quantitative RT-PCR was positive for CHIKV in 54 patients. The median copy number of CHIKV was 1.3x 108 copies/ml (1.7x105-9.9x109 copies/ml). Among the observed clinical features, a statistically significant difference in log mean virus load was found between patients with and without myalgia (log mean 7.50 vs 8.34, P = 0.01). Patients with myalgia had lower virus load and those without myalgia had a higher virus load.