Presence and function of stress granules in atrial fibrillation

• Published in: PloS one 2019-04, Vol.14 (4), p.e0213769-e0213769
• Main Authors: Dong, Guo, Liang, Fengying, Sun, Bo, Wang, Chengcheng, Liu, Yangyang, Guan, Xiangpeng, Yang, Bo, Xiu, Chunhong, Yang, Ning, Liu, Fengyu, Lu, Tianyi, Han, Wei
• Format: Article
• Language: English
• Subjects: Angiotensin; Angiotensin II; Angiotensins; Animal models; Animals; Antioxidants; Apoptosis; Atrial fibrillation; Atrial Fibrillation - pathology; Biology and Life Sciences; Calcium; Cardiac arrhythmia; Cardiology; Cardiomyocytes; Care and treatment; Cell culture; Cell Line; Cell lines; Cell Proliferation; Collagen; Collagen (type I); Cytoplasmic Granules - physiology; Disease Models, Animal; DNA Helicases - metabolism; Electron microscopy; Fibrillation; Fibroblasts; Fibroblasts - cytology; Fibroblasts - physiology; Fibronectin; Fibronectins; Fibrosis; Flow cytometry; Fluorescent antibody technique; Granular materials; GTPase-activating protein; Guanosine triphosphatases; Heart; Heart Atria - cytology; Heart Atria - pathology; Heart cells; Humans; Immunofluorescence; Inflammation; Kinases; Low level; Medicine and Health Sciences; Messenger RNA; Mice; MicroRNAs; Microscopy; mRNA; Myocytes, Cardiac - cytology; Myocytes, Cardiac - physiology; Oxidative stress; Oxidative Stress - physiology; Oxygen; Pathogenesis; Poly(A)-binding protein; Poly-ADP-Ribose Binding Proteins - metabolism; Polyadenine; Primary Cell Culture; Protein binding; Protein biosynthesis; Protein synthesis; Proteins; Rats; Reactive oxygen species; Research and Analysis Methods; RNA; RNA Helicases - metabolism; RNA Recognition Motif Proteins - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0213769

Stress granules (SGs) are transient cytoplasmic mRNA and protein complexes that form in eukaryotic cells under stress. SGs are related to multiple diseases, but there are no reports of the existence of SGs in atrial fibrillation (AF). Cell models of AF were established by field stimulation at 600 times per minute. HL-1 cells, cardiomyocytes and cardiac fibroblasts were transfected with G3BP1-cDNA plasmid by Lipofectamine 2000. The presence of SGs was detected by immunofluorescence analysis against GTPase-activating protein SH3 domain binding protein 1 (G3BP1) and poly(A)-binding protein 1 (PABP-1) and electron microscopy. Stable HL-1 cell lines transfected with lentivirus overexpressing G3BP1were constructed to further induce the formation of SGs in AF. Reactive oxygen species (ROS) and calcium overload in tachypaced HL-1 cells were studied by flow cytometry. The effects of G3BP1 overexpression on cardiac fibroblast proliferation and the protein expression level of collagen I/III and fibronectin-1 were also studied. Additionally, we detected protein synthesis in general and in single cells by puromycin incorporation in paced HL-1 cells. Here, we first showed that SGs are present in both tachypaced mouse cardiomyocytes and HL-1 atrial cells, although the presence is partial and at a low level. G3BP1 overexpression promoted SG formation, inhibited the rapid pacing-induced increase in ROS level, and attenuated calcium overload in HL-1 cells (all P