PBMCs transcriptome profiles identified breed-specific transcriptome signatures for PRRSV vaccination in German Landrace and Pietrain pigs

• Published in: PloS one 2019-09, Vol.14 (9), p.e0222513-e0222513
• Main Authors: Islam, Md Aminul, Neuhoff, Christiane, Aqter Rony, Sharmin, Große-Brinkhaus, Christine, Uddin, Muhammad Jasim, Hölker, Michael, Tesfaye, Dawit, Tholen, Ernst, Schellander, Karl, Pröll-Cornelissen, Maren Julia
• Format: Article
• Language: English
• Subjects: Agriculture; Analysis; Animal breeding; Animal sciences; Animals; Antibodies, Viral - immunology; Antigens; Biology and Life Sciences; Blood cells; Breeding - methods; Breeding of animals; Cancer; Computer and Information Sciences; Disease; DNA microarrays; Drug resistance; FOXO3 protein; Gene expression; Gene Expression - genetics; Gene Expression - immunology; Genes; Genetic aspects; Genetic diversity; Genetic variation; Genomics; Hogs; Immune response; Immune system; Immunity, Innate - genetics; Immunity, Innate - immunology; Immunocompetence; Infections; Innate immunity; Kinases; Landraces; Leukocytes (mononuclear); Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - physiology; Leukocytes, Mononuclear - virology; Livestock; Medicine and Health Sciences; Nidoviruses; Outcome and process assessment (Medical care); Peripheral blood mononuclear cells; Porcine reproductive and respiratory syndrome; Porcine Reproductive and Respiratory Syndrome - immunology; Porcine Reproductive and Respiratory Syndrome - virology; Porcine respiratory and reproductive syndrome virus - immunology; Pork industry; Proteins; Response functions; Stat1 protein; Swine; Transcription factors; Transcriptome - genetics; Transcriptome - immunology; Vaccination; Vaccination - methods; Vaccines; Viral diseases; Viral infections; Viral Vaccines - immunology; Zoology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0222513

Porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting the swine industry worldwide. Genetic variation in host immunity has been considered as one of the potential determinants to improve the immunocompetence, thereby resistance to PRRS. Therefore, the present study aimed to investigate the breed difference in innate immune response to PRRSV vaccination between German Landrace (DL) and Pietrain (Pi) pigs. We analyzed microarray-based transcriptome profiles of peripheral blood mononuclear cells (PBMCs) collected before (0 h) and 24 h after PRRSV vaccination from purebred DL and Pi pigs with three biological replicates. In total 4,269 transcripts were identified to be differentially expressed in PBMCs in at least any of four tested contrast pairs (i.e. DL-24h vs. DL-0h, Pi-24h vs. Pi-0h, DL-0h vs. Pi-0h and DL-24h vs. Pi-24h). The number of vaccine-induced differentially expressed genes (DEGs) was much higher (2,459) in DL pigs than that of Pi pigs (291). After 24 h of PRRSV vaccination, 1,046 genes were differentially expressed in PMBCs of DL pigs compared to that of Pi (DL-24h vs. Pi-24h), indicating the breed differences in vaccine responsiveness. The top biological pathways significantly affected by DEGs of both breeds were linked to immune response functions. The network enrichment analysis identified ADAM17, STAT1, MMS19, RPA2, BAD, UCHL5 and APC as potential regulatory genes for the functional network of PRRSV vaccine response specific for DL; while FOXO3, IRF2, ADRBK1, FHL3, PPP2CB and NCOA6 were found to be the most potential hubs of Pi specific transcriptome network. In conclusion, our data provided insights of breed-specific host transcriptome responses to PRRSV vaccination which might contribute in better understanding of PPRS resistance in pigs.