Micro-RNA signatures in monozygotic twins discordant for congenital heart defects

MicroRNAs (miRNAs) are small RNAs regulating gene expression post-transcriptionally. Recent studies demonstrated that miRNAs are involved in the development of congenital heart defects (CHD). In this study, we aimed at identifying the specific patterns of miRNAs in blood of monozygotic twin pairs di...

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Published in:PloS one 2019-12, Vol.14 (12), p.e0226164-e0226164
Main Authors: Abu-Halima, Masood, Weidinger, Josephin, Poryo, Martin, Henn, Dominic, Keller, Andreas, Meese, Eckart, Abdul-Khaliq, Hashim
Format: Article
Language:English
Subjects:
Abundance
Adolescent
Bioinformatics
Biology and life sciences
Blood
Cardiology
Cardiomyocytes
Case-Control Studies
Child
Defects
Deoxyribonucleic acid
Deregulation
Diseases in Twins - genetics
DNA
DNA microarrays
Female
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation
Heart attacks
Heart Defects, Congenital - genetics
Heart failure
Humans
Medicine and Health Sciences
MicroRNAs
MicroRNAs - genetics
miRNA
Oligonucleotide Array Sequence Analysis
Pediatrics
Peripheral blood
Phosphatidylinositol
Post-transcription
Research and Analysis Methods
Ribonucleic acid
RNA
Sequence Analysis, RNA
Signaling
Twins
Twins, Monozygotic - genetics
Young Adult
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cited_by cdi_FETCH-LOGICAL-c526t-e19208f27d8442bd8ce7ca8f254d5c742bc33faa89a7de80dd4a7e607dda6b443
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container_title PloS one
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creator Abu-Halima, Masood
Weidinger, Josephin
Poryo, Martin
Henn, Dominic
Keller, Andreas
Meese, Eckart
Abdul-Khaliq, Hashim
description MicroRNAs (miRNAs) are small RNAs regulating gene expression post-transcriptionally. Recent studies demonstrated that miRNAs are involved in the development of congenital heart defects (CHD). In this study, we aimed at identifying the specific patterns of miRNAs in blood of monozygotic twin pairs discordant for CHD and to assess whether miRNAs might be involved in the development or reflect the consequences of CHD. miRNA microarray analysis and Real-Time Quantitative PCR (RT-qPCR) were employed to determine the miRNA abundance level from 12 monozygotic twins discordant for CHD and their non-CHD co-twins (n = 12). Enrichment analyses of altered miRNAs were performed using bioinformatics tools. Compared with non-CHD co-twins, profiling analysis indicated 34 miRNAs with a significant difference in abundance level (p
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Recent studies demonstrated that miRNAs are involved in the development of congenital heart defects (CHD). In this study, we aimed at identifying the specific patterns of miRNAs in blood of monozygotic twin pairs discordant for CHD and to assess whether miRNAs might be involved in the development or reflect the consequences of CHD. miRNA microarray analysis and Real-Time Quantitative PCR (RT-qPCR) were employed to determine the miRNA abundance level from 12 monozygotic twins discordant for CHD and their non-CHD co-twins (n = 12). Enrichment analyses of altered miRNAs were performed using bioinformatics tools. Compared with non-CHD co-twins, profiling analysis indicated 34 miRNAs with a significant difference in abundance level (p&lt;0.05, fold change ≥ 1.3), of which 11 miRNAs were up-regulated and 23 miRNAs were down-regulated. Seven miRNAs were validated with RT-qPCR including miR-511-3p, miR-1306-5p, miR-421, miR-4707-3p, miR-4732-3p, miR-5189-3p, and miR-890, and the results were consistent with microarray analysis. Five miRNAs namely miR-511-3p, miR-1306-5p, miR-4732-3p, miR-5189-3p, and miR-890 were found to be significantly up-regulated in twins &lt; 10 years old. Bioinformatics analysis showed that the 7 validated miRNAs were involved in phosphatidylinositol signaling, gap junction signaling, and adrenergic signaling in cardiomyocytes. Our data show deregulated miRNA abundance levels in the peripheral blood of monozygotic twins discordant for CHD, and identify new candidates for further analysis, which may contribute to understanding the development of CHD in the future. 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Recent studies demonstrated that miRNAs are involved in the development of congenital heart defects (CHD). In this study, we aimed at identifying the specific patterns of miRNAs in blood of monozygotic twin pairs discordant for CHD and to assess whether miRNAs might be involved in the development or reflect the consequences of CHD. miRNA microarray analysis and Real-Time Quantitative PCR (RT-qPCR) were employed to determine the miRNA abundance level from 12 monozygotic twins discordant for CHD and their non-CHD co-twins (n = 12). Enrichment analyses of altered miRNAs were performed using bioinformatics tools. Compared with non-CHD co-twins, profiling analysis indicated 34 miRNAs with a significant difference in abundance level (p&lt;0.05, fold change ≥ 1.3), of which 11 miRNAs were up-regulated and 23 miRNAs were down-regulated. Seven miRNAs were validated with RT-qPCR including miR-511-3p, miR-1306-5p, miR-421, miR-4707-3p, miR-4732-3p, miR-5189-3p, and miR-890, and the results were consistent with microarray analysis. Five miRNAs namely miR-511-3p, miR-1306-5p, miR-4732-3p, miR-5189-3p, and miR-890 were found to be significantly up-regulated in twins &lt; 10 years old. Bioinformatics analysis showed that the 7 validated miRNAs were involved in phosphatidylinositol signaling, gap junction signaling, and adrenergic signaling in cardiomyocytes. Our data show deregulated miRNA abundance levels in the peripheral blood of monozygotic twins discordant for CHD, and identify new candidates for further analysis, which may contribute to understanding the development of CHD in the future. Bioinformatics analysis indicated that the target genes of these miRNAs are likely involved in signaling and communication of cardiomyocytes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31805172</pmid><doi>10.1371/journal.pone.0226164</doi><orcidid>https://orcid.org/0000-0001-9048-4958</orcidid><oa>free_for_read</oa></addata></record>
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subjects Abundance
Adolescent
Bioinformatics
Biology and life sciences
Blood
Cardiology
Cardiomyocytes
Case-Control Studies
Child
Defects
Deoxyribonucleic acid
Deregulation
Diseases in Twins - genetics
DNA
DNA microarrays
Female
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation
Heart attacks
Heart Defects, Congenital - genetics
Heart failure
Humans
Medicine and Health Sciences
MicroRNAs
MicroRNAs - genetics
miRNA
Oligonucleotide Array Sequence Analysis
Pediatrics
Peripheral blood
Phosphatidylinositol
Post-transcription
Research and Analysis Methods
Ribonucleic acid
RNA
Sequence Analysis, RNA
Signaling
Twins
Twins, Monozygotic - genetics
Young Adult
title Micro-RNA signatures in monozygotic twins discordant for congenital heart defects
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