Comprehensive proteomics and functional annotation of mouse brown adipose tissue

Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of the function of mammalian BAT. Herein, a comprehensive analysis of interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct a comprehensive...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2020-05, Vol.15 (5), p.e0232084-e0232084
Main Authors: Li, Jing, Li, Juan, Zhao, Wei-Gang, Sun, Hai-Dan, Guo, Zheng-Guang, Liu, Xiao-Yan, Tang, Xiao-Yue, She, Zhu-Fang, Yuan, Tao, Liu, Shuai-Nan, Liu, Quan, Fu, Yong, Sun, Wei
Format: Article
Language:English
Subjects:
Adenosine
Adipocytes
Adipose tissue
Adipose tissue (brown)
Adipose Tissue, Brown - chemistry
Adipose Tissue, Brown - metabolism
Animal tissues
Animals
Annotations
Apoptosis
Biology and Life Sciences
Biomarkers
Body fat
Chromatography
Chromatography, High Pressure Liquid
Chromatography, Liquid
Cytokines
Databases, Protein
Diabetes
Endocrinology
Fatty acids
Female
Future predictions
Glucose
Hospitals
House mouse
Kinases
Laboratories
Mammals
Mass spectrometry
Mass spectroscopy
Medical schools
Medicine
Medicine and Health Sciences
Methods
Mice
Mice, Inbred C57BL
Molecular modelling
Molecular Sequence Annotation - methods
Oxidation
Oxidation-reduction reactions
Peptides
Phosphorylation
Physiological aspects
Protein biosynthesis
Protein synthesis
Proteins
Proteome - analysis
Proteome - metabolism
Proteomes
Proteomics
Proteomics - methods
R&D
Research & development
Scientific imaging
Signal peptides
Spectroscopy
Studies
Tandem Mass Spectrometry
Thermogenesis
Thermoregulation
Time
Type 2 diabetes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c692t-3334e26ae53f7548ad8678ce48f5f88be58e2973b5d997136a5c950e8ff9093f3
cites cdi_FETCH-LOGICAL-c692t-3334e26ae53f7548ad8678ce48f5f88be58e2973b5d997136a5c950e8ff9093f3
container_end_page e0232084
container_issue 5
container_start_page e0232084
container_title PloS one
container_volume 15
creator Li, Jing
Li, Juan
Zhao, Wei-Gang
Sun, Hai-Dan
Guo, Zheng-Guang
Liu, Xiao-Yan
Tang, Xiao-Yue
She, Zhu-Fang
Yuan, Tao
Liu, Shuai-Nan
Liu, Quan
Fu, Yong
Sun, Wei
description Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of the function of mammalian BAT. Herein, a comprehensive analysis of interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct a comprehensive proteome dataset of mouse BAT proteins. A total of 4949 nonredundant proteins were identified, and 4495 were quantified using the iBAQ method. According to the iBAQ values, the BAT proteome was divided into high-, middle- and low-abundance proteins. The functions of the high-abundance proteins were mainly related to glucose and fatty acid oxidation to produce heat for thermoregulation, while the functions of the middle- and low-abundance proteins were mainly related to protein synthesis and apoptosis, respectively. Additionally, 497 proteins were predicted to have signal peptides using SignalP4 software, and 75 were confirmed in previous studies. This study, for the first time, comprehensively profiled and functionally annotated the BAT proteome. This study will be helpful for future studies focused on biomarker identification and BAT molecular mechanisms.
doi_str_mv 10.1371/journal.pone.0232084
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2399252433</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A622865108</galeid><doaj_id>oai_doaj_org_article_256b8764b84045e9b40d45e91ce17585</doaj_id><sourcerecordid>A622865108</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-3334e26ae53f7548ad8678ce48f5f88be58e2973b5d997136a5c950e8ff9093f3</originalsourceid><addsrcrecordid>eNqNkk1r3DAQhk1paZJt_0FpDYXSHnYrS5YsXQph6cdCIKVfVyHbo10ttuVIcpr--8pZJ6xLDkUHaeRn3rFm3iR5kaFVRors_d4OrlPNqrcdrBAmGPH8UXKaCYKXDCPy-Oh8kpx5v0eIEs7Y0-SEYFLkBaGnyde1bXsHO-i8uYa0dzaAbU3lU9XVqR66Khgby8Sws0GNQWp12trBQ1o6-7tLVW16G6NgvB_gWfJEq8bD82lfJD8_ffyx_rK8uPy8WZ9fLCsmcFgSQnLATAEluqA5VzVnBa8g55pqzkugHLAoSElrIYqMMEUrQRFwrQUSRJNF8uqg2zfWy6kZXmIiBKY4j_qLZHMgaqv2snemVe6PtMrI2wvrtlK5YKoGJKas5AXLS56jnIIoc1SPe1ZBVlBOo9aHqdpQtlBX0AWnmpno_EtndnJrr2WBEWZxOovk7STg7NUAPsjW-AqaRnUQe3n735zwjI21Xv-DPvy6idqq-ADTaRvrVqOoPGcYc0YzxCO1eoCKq4Y45OgcbeL9LOHdLCEyAW7CVg3ey833b__PXv6as2-O2B2oJuy8bYbRUH4O5gewctZ7B_q-yRmSo_HvuiFH48vJ-DHt5fGA7pPunE7-Aie4_OQ</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2399252433</pqid></control><display><type>article</type><title>Comprehensive proteomics and functional annotation of mouse brown adipose tissue</title><source>Publicly Available Content (ProQuest)</source><source>PubMed Central</source><creator>Li, Jing ; Li, Juan ; Zhao, Wei-Gang ; Sun, Hai-Dan ; Guo, Zheng-Guang ; Liu, Xiao-Yan ; Tang, Xiao-Yue ; She, Zhu-Fang ; Yuan, Tao ; Liu, Shuai-Nan ; Liu, Quan ; Fu, Yong ; Sun, Wei</creator><contributor>Peterson, Jonathan M</contributor><creatorcontrib>Li, Jing ; Li, Juan ; Zhao, Wei-Gang ; Sun, Hai-Dan ; Guo, Zheng-Guang ; Liu, Xiao-Yan ; Tang, Xiao-Yue ; She, Zhu-Fang ; Yuan, Tao ; Liu, Shuai-Nan ; Liu, Quan ; Fu, Yong ; Sun, Wei ; Peterson, Jonathan M</creatorcontrib><description>Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of the function of mammalian BAT. Herein, a comprehensive analysis of interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct a comprehensive proteome dataset of mouse BAT proteins. A total of 4949 nonredundant proteins were identified, and 4495 were quantified using the iBAQ method. According to the iBAQ values, the BAT proteome was divided into high-, middle- and low-abundance proteins. The functions of the high-abundance proteins were mainly related to glucose and fatty acid oxidation to produce heat for thermoregulation, while the functions of the middle- and low-abundance proteins were mainly related to protein synthesis and apoptosis, respectively. Additionally, 497 proteins were predicted to have signal peptides using SignalP4 software, and 75 were confirmed in previous studies. This study, for the first time, comprehensively profiled and functionally annotated the BAT proteome. This study will be helpful for future studies focused on biomarker identification and BAT molecular mechanisms.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0232084</identifier><identifier>PMID: 32374735</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenosine ; Adipocytes ; Adipose tissue ; Adipose tissue (brown) ; Adipose Tissue, Brown - chemistry ; Adipose Tissue, Brown - metabolism ; Animal tissues ; Animals ; Annotations ; Apoptosis ; Biology and Life Sciences ; Biomarkers ; Body fat ; Chromatography ; Chromatography, High Pressure Liquid ; Chromatography, Liquid ; Cytokines ; Databases, Protein ; Diabetes ; Endocrinology ; Fatty acids ; Female ; Future predictions ; Glucose ; Hospitals ; House mouse ; Kinases ; Laboratories ; Mammals ; Mass spectrometry ; Mass spectroscopy ; Medical schools ; Medicine ; Medicine and Health Sciences ; Methods ; Mice ; Mice, Inbred C57BL ; Molecular modelling ; Molecular Sequence Annotation - methods ; Oxidation ; Oxidation-reduction reactions ; Peptides ; Phosphorylation ; Physiological aspects ; Protein biosynthesis ; Protein synthesis ; Proteins ; Proteome - analysis ; Proteome - metabolism ; Proteomes ; Proteomics ; Proteomics - methods ; R&amp;D ; Research &amp; development ; Scientific imaging ; Signal peptides ; Spectroscopy ; Studies ; Tandem Mass Spectrometry ; Thermogenesis ; Thermoregulation ; Time ; Type 2 diabetes</subject><ispartof>PloS one, 2020-05, Vol.15 (5), p.e0232084-e0232084</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Li et al 2020 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-3334e26ae53f7548ad8678ce48f5f88be58e2973b5d997136a5c950e8ff9093f3</citedby><cites>FETCH-LOGICAL-c692t-3334e26ae53f7548ad8678ce48f5f88be58e2973b5d997136a5c950e8ff9093f3</cites><orcidid>0000-0003-1120-6832</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2399252433/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2399252433?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32374735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Peterson, Jonathan M</contributor><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Li, Juan</creatorcontrib><creatorcontrib>Zhao, Wei-Gang</creatorcontrib><creatorcontrib>Sun, Hai-Dan</creatorcontrib><creatorcontrib>Guo, Zheng-Guang</creatorcontrib><creatorcontrib>Liu, Xiao-Yan</creatorcontrib><creatorcontrib>Tang, Xiao-Yue</creatorcontrib><creatorcontrib>She, Zhu-Fang</creatorcontrib><creatorcontrib>Yuan, Tao</creatorcontrib><creatorcontrib>Liu, Shuai-Nan</creatorcontrib><creatorcontrib>Liu, Quan</creatorcontrib><creatorcontrib>Fu, Yong</creatorcontrib><creatorcontrib>Sun, Wei</creatorcontrib><title>Comprehensive proteomics and functional annotation of mouse brown adipose tissue</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of the function of mammalian BAT. Herein, a comprehensive analysis of interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct a comprehensive proteome dataset of mouse BAT proteins. A total of 4949 nonredundant proteins were identified, and 4495 were quantified using the iBAQ method. According to the iBAQ values, the BAT proteome was divided into high-, middle- and low-abundance proteins. The functions of the high-abundance proteins were mainly related to glucose and fatty acid oxidation to produce heat for thermoregulation, while the functions of the middle- and low-abundance proteins were mainly related to protein synthesis and apoptosis, respectively. Additionally, 497 proteins were predicted to have signal peptides using SignalP4 software, and 75 were confirmed in previous studies. This study, for the first time, comprehensively profiled and functionally annotated the BAT proteome. This study will be helpful for future studies focused on biomarker identification and BAT molecular mechanisms.</description><subject>Adenosine</subject><subject>Adipocytes</subject><subject>Adipose tissue</subject><subject>Adipose tissue (brown)</subject><subject>Adipose Tissue, Brown - chemistry</subject><subject>Adipose Tissue, Brown - metabolism</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Annotations</subject><subject>Apoptosis</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Body fat</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Liquid</subject><subject>Cytokines</subject><subject>Databases, Protein</subject><subject>Diabetes</subject><subject>Endocrinology</subject><subject>Fatty acids</subject><subject>Female</subject><subject>Future predictions</subject><subject>Glucose</subject><subject>Hospitals</subject><subject>House mouse</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Mammals</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical schools</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular modelling</subject><subject>Molecular Sequence Annotation - methods</subject><subject>Oxidation</subject><subject>Oxidation-reduction reactions</subject><subject>Peptides</subject><subject>Phosphorylation</subject><subject>Physiological aspects</subject><subject>Protein biosynthesis</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Proteome - analysis</subject><subject>Proteome - metabolism</subject><subject>Proteomes</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><subject>R&amp;D</subject><subject>Research &amp; development</subject><subject>Scientific imaging</subject><subject>Signal peptides</subject><subject>Spectroscopy</subject><subject>Studies</subject><subject>Tandem Mass Spectrometry</subject><subject>Thermogenesis</subject><subject>Thermoregulation</subject><subject>Time</subject><subject>Type 2 diabetes</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNkk1r3DAQhk1paZJt_0FpDYXSHnYrS5YsXQph6cdCIKVfVyHbo10ttuVIcpr--8pZJ6xLDkUHaeRn3rFm3iR5kaFVRors_d4OrlPNqrcdrBAmGPH8UXKaCYKXDCPy-Oh8kpx5v0eIEs7Y0-SEYFLkBaGnyde1bXsHO-i8uYa0dzaAbU3lU9XVqR66Khgby8Sws0GNQWp12trBQ1o6-7tLVW16G6NgvB_gWfJEq8bD82lfJD8_ffyx_rK8uPy8WZ9fLCsmcFgSQnLATAEluqA5VzVnBa8g55pqzkugHLAoSElrIYqMMEUrQRFwrQUSRJNF8uqg2zfWy6kZXmIiBKY4j_qLZHMgaqv2snemVe6PtMrI2wvrtlK5YKoGJKas5AXLS56jnIIoc1SPe1ZBVlBOo9aHqdpQtlBX0AWnmpno_EtndnJrr2WBEWZxOovk7STg7NUAPsjW-AqaRnUQe3n735zwjI21Xv-DPvy6idqq-ADTaRvrVqOoPGcYc0YzxCO1eoCKq4Y45OgcbeL9LOHdLCEyAW7CVg3ey833b__PXv6as2-O2B2oJuy8bYbRUH4O5gewctZ7B_q-yRmSo_HvuiFH48vJ-DHt5fGA7pPunE7-Aie4_OQ</recordid><startdate>20200506</startdate><enddate>20200506</enddate><creator>Li, Jing</creator><creator>Li, Juan</creator><creator>Zhao, Wei-Gang</creator><creator>Sun, Hai-Dan</creator><creator>Guo, Zheng-Guang</creator><creator>Liu, Xiao-Yan</creator><creator>Tang, Xiao-Yue</creator><creator>She, Zhu-Fang</creator><creator>Yuan, Tao</creator><creator>Liu, Shuai-Nan</creator><creator>Liu, Quan</creator><creator>Fu, Yong</creator><creator>Sun, Wei</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1120-6832</orcidid></search><sort><creationdate>20200506</creationdate><title>Comprehensive proteomics and functional annotation of mouse brown adipose tissue</title><author>Li, Jing ; Li, Juan ; Zhao, Wei-Gang ; Sun, Hai-Dan ; Guo, Zheng-Guang ; Liu, Xiao-Yan ; Tang, Xiao-Yue ; She, Zhu-Fang ; Yuan, Tao ; Liu, Shuai-Nan ; Liu, Quan ; Fu, Yong ; Sun, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-3334e26ae53f7548ad8678ce48f5f88be58e2973b5d997136a5c950e8ff9093f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adenosine</topic><topic>Adipocytes</topic><topic>Adipose tissue</topic><topic>Adipose tissue (brown)</topic><topic>Adipose Tissue, Brown - chemistry</topic><topic>Adipose Tissue, Brown - metabolism</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Annotations</topic><topic>Apoptosis</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Body fat</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Liquid</topic><topic>Cytokines</topic><topic>Databases, Protein</topic><topic>Diabetes</topic><topic>Endocrinology</topic><topic>Fatty acids</topic><topic>Female</topic><topic>Future predictions</topic><topic>Glucose</topic><topic>Hospitals</topic><topic>House mouse</topic><topic>Kinases</topic><topic>Laboratories</topic><topic>Mammals</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical schools</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular modelling</topic><topic>Molecular Sequence Annotation - methods</topic><topic>Oxidation</topic><topic>Oxidation-reduction reactions</topic><topic>Peptides</topic><topic>Phosphorylation</topic><topic>Physiological aspects</topic><topic>Protein biosynthesis</topic><topic>Protein synthesis</topic><topic>Proteins</topic><topic>Proteome - analysis</topic><topic>Proteome - metabolism</topic><topic>Proteomes</topic><topic>Proteomics</topic><topic>Proteomics - methods</topic><topic>R&amp;D</topic><topic>Research &amp; development</topic><topic>Scientific imaging</topic><topic>Signal peptides</topic><topic>Spectroscopy</topic><topic>Studies</topic><topic>Tandem Mass Spectrometry</topic><topic>Thermogenesis</topic><topic>Thermoregulation</topic><topic>Time</topic><topic>Type 2 diabetes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Li, Juan</creatorcontrib><creatorcontrib>Zhao, Wei-Gang</creatorcontrib><creatorcontrib>Sun, Hai-Dan</creatorcontrib><creatorcontrib>Guo, Zheng-Guang</creatorcontrib><creatorcontrib>Liu, Xiao-Yan</creatorcontrib><creatorcontrib>Tang, Xiao-Yue</creatorcontrib><creatorcontrib>She, Zhu-Fang</creatorcontrib><creatorcontrib>Yuan, Tao</creatorcontrib><creatorcontrib>Liu, Shuai-Nan</creatorcontrib><creatorcontrib>Liu, Quan</creatorcontrib><creatorcontrib>Fu, Yong</creatorcontrib><creatorcontrib>Sun, Wei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale in Context : Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing &amp; Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jing</au><au>Li, Juan</au><au>Zhao, Wei-Gang</au><au>Sun, Hai-Dan</au><au>Guo, Zheng-Guang</au><au>Liu, Xiao-Yan</au><au>Tang, Xiao-Yue</au><au>She, Zhu-Fang</au><au>Yuan, Tao</au><au>Liu, Shuai-Nan</au><au>Liu, Quan</au><au>Fu, Yong</au><au>Sun, Wei</au><au>Peterson, Jonathan M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comprehensive proteomics and functional annotation of mouse brown adipose tissue</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-05-06</date><risdate>2020</risdate><volume>15</volume><issue>5</issue><spage>e0232084</spage><epage>e0232084</epage><pages>e0232084-e0232084</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of the function of mammalian BAT. Herein, a comprehensive analysis of interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct a comprehensive proteome dataset of mouse BAT proteins. A total of 4949 nonredundant proteins were identified, and 4495 were quantified using the iBAQ method. According to the iBAQ values, the BAT proteome was divided into high-, middle- and low-abundance proteins. The functions of the high-abundance proteins were mainly related to glucose and fatty acid oxidation to produce heat for thermoregulation, while the functions of the middle- and low-abundance proteins were mainly related to protein synthesis and apoptosis, respectively. Additionally, 497 proteins were predicted to have signal peptides using SignalP4 software, and 75 were confirmed in previous studies. This study, for the first time, comprehensively profiled and functionally annotated the BAT proteome. This study will be helpful for future studies focused on biomarker identification and BAT molecular mechanisms.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>32374735</pmid><doi>10.1371/journal.pone.0232084</doi><tpages>e0232084</tpages><orcidid>https://orcid.org/0000-0003-1120-6832</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2020-05, Vol.15 (5), p.e0232084-e0232084
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_2399252433
source Publicly Available Content (ProQuest); PubMed Central
subjects Adenosine
Adipocytes
Adipose tissue
Adipose tissue (brown)
Adipose Tissue, Brown - chemistry
Adipose Tissue, Brown - metabolism
Animal tissues
Animals
Annotations
Apoptosis
Biology and Life Sciences
Biomarkers
Body fat
Chromatography
Chromatography, High Pressure Liquid
Chromatography, Liquid
Cytokines
Databases, Protein
Diabetes
Endocrinology
Fatty acids
Female
Future predictions
Glucose
Hospitals
House mouse
Kinases
Laboratories
Mammals
Mass spectrometry
Mass spectroscopy
Medical schools
Medicine
Medicine and Health Sciences
Methods
Mice
Mice, Inbred C57BL
Molecular modelling
Molecular Sequence Annotation - methods
Oxidation
Oxidation-reduction reactions
Peptides
Phosphorylation
Physiological aspects
Protein biosynthesis
Protein synthesis
Proteins
Proteome - analysis
Proteome - metabolism
Proteomes
Proteomics
Proteomics - methods
R&D
Research & development
Scientific imaging
Signal peptides
Spectroscopy
Studies
Tandem Mass Spectrometry
Thermogenesis
Thermoregulation
Time
Type 2 diabetes
title Comprehensive proteomics and functional annotation of mouse brown adipose tissue
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T06%3A59%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comprehensive%20proteomics%20and%20functional%20annotation%20of%20mouse%20brown%20adipose%20tissue&rft.jtitle=PloS%20one&rft.au=Li,%20Jing&rft.date=2020-05-06&rft.volume=15&rft.issue=5&rft.spage=e0232084&rft.epage=e0232084&rft.pages=e0232084-e0232084&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0232084&rft_dat=%3Cgale_plos_%3EA622865108%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-3334e26ae53f7548ad8678ce48f5f88be58e2973b5d997136a5c950e8ff9093f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2399252433&rft_id=info:pmid/32374735&rft_galeid=A622865108&rfr_iscdi=true