Molecular characterization of Campylobacter spp. recovered from beef, chicken, lamb and pork products at retail in Australia

Australian rates of campylobacteriosis are among the highest in developed countries, yet only limited work has been done to characterize Campylobacter spp. in Australian retail products. We performed whole genome sequencing (WGS) on 331 C. coli and 285 C. jejuni from retail chicken meat, as well as...

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Bibliographic Details
Published in:PloS one 2020-07, Vol.15 (7), p.e0236889
Main Authors: Wallace, Rhiannon L., Bulach, Dieter M., Jennison, Amy V., Valcanis, Mary, McLure, Angus, Smith, James J., Graham, Trudy, Saputra, Themy, Firestone, Simon, Symes, Sally, Waters, Natasha, Stylianopoulos, Anastasia, Kirk, Martyn D., Glass, Kathryn
Format: Article
Language:English
Subjects:
Amides
Aminoglycoside antibiotics
Aminoglycosides
Antibiotics
Antimicrobial agents
Antimicrobial resistance
Beef
Biology and Life Sciences
Campylobacter
Campylobacteriosis
Chickens
Deoxyribonucleic acid
Developed countries
DNA
DNA topoisomerase
Epidemiology
Food
Gene sequencing
Genomes
Genomic analysis
Genotypes
Guillain-Barre syndrome
Laboratories
Lincosamides
Meat
Medicine and Health Sciences
Methods
Microorganisms
Multidrug resistance
Mutation
Organs
Pathogens
Population
Pork
Poultry
Public health
State government
Whole genome sequencing
β-Lactam antibiotics
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Summary:Australian rates of campylobacteriosis are among the highest in developed countries, yet only limited work has been done to characterize Campylobacter spp. in Australian retail products. We performed whole genome sequencing (WGS) on 331 C. coli and 285 C. jejuni from retail chicken meat, as well as beef, chicken, lamb and pork offal (organs). Campylobacter isolates were highly diverse, with 113 sequence types (STs) including 38 novel STs, identified from 616 isolates. Genomic analysis suggests very low levels (2.3–15.3%) of resistance to aminoglycoside, beta-lactam, fluoroquinolone, macrolide and tetracycline antibiotics. A majority (>90%) of isolates (52/56) possessing the fluoroquinolone resistance-associated T86I mutation in the gyrA gene belonged to ST860, ST2083 or ST7323. The 44 pork offal isolates were highly diverse, representing 33 STs (11 novel STs) and harboured genes associated with resistance to aminoglycosides, lincosamides and macrolides not generally found in isolates from other sources. Prevalence of multidrug resistant genotypes was very low (
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0236889