Molecular characterization of invasive Enterobacteriaceae from pediatric patients in Central and Northwestern Nigeria

• Published in: PloS one 2020-10, Vol.15 (10), p.e0230037-e0230037
• Main Authors: Duru, Carissa, Olanipekun, Grace, Odili, Vivian, Kocmich, Nicholas, Rezac, Amy, Ajose, Theresa O, Medugu, Nubwa, Umoru, Dominic, Onuchukwu, Chukwuma, Munir, Huda, Jibir, Binta Wudil, Farouk, Zubaida, Gambo, Safiya, Hassan-Hanga, Fatimah, Olaosebikan, Rasaq, Ebruke, Bernard, Esimone, Charles, Obaro, Stephen
• Format: Article
• Language: English
• Subjects: Amikacin; Antibiotics; Antimicrobial agents; Automation; Aztreonam; Bacteremia; Bacteria; Biology and Life Sciences; Blood; Blood culture; Cefotaxime; Cefpodoxime; Ceftriaxone; Children; Deoxyribonucleic acid; Developing countries; DNA; Drug resistance; Dynamic tests; E coli; Enterobacteriaceae; Enzymes; Etiology; Genes; Genetic aspects; Health aspects; Health care facilities; High resistance; Imipenem; Infections; Infectious diseases; Laboratories; LDCs; Medicine and Health Sciences; Meropenem; Mutation; Pediatrics; People and Places; Research and Analysis Methods; Salmonella; Sepsis; Sick children; Statistical analysis; Sulfamethoxazole; Teaching hospitals; Trimethoprim
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0230037

Bacteremia is a leading cause of mortality in developing countries, however, etiologic evaluation is infrequent and empiric antibiotic use not evidence-based. Here, we evaluated the patterns of ESBL resistance in children enrolled into a surveillance study for community acquired bacteremic syndromes across health facilities in Central and Northwestern Nigeria. Blood culture was performed for children aged less than 5 years suspected of having sepsis from Sept 2008-Dec 2016. Blood was incubated using the BACTEC.sup.00AE system and Enterobacteriacea identified to the species level using Analytical Profile Index (API20E.sup.®). Antibiotic susceptibility profile was determined by the disc diffusion method. Real time PCR was used to characterize genes responsible for ESBL production. Of 21,000 children screened from Sept 2008-Dec 2016, 2,625(12.5%) were culture-positive. A total of 413 Enterobacteriaceae available for analysis were screened for ESBL. ESBL production was detected in 160 Enterobacteriaceae, high resistance rates were observed among ESBL-positive isolates for Ceftriaxone (92.3%), Aztreonam (96.8%), Cefpodoxime (96.3%), Cefotaxime (98.8%) and Trimethoprim/sulfamethoxazole (90%), while 87.5%, 90.7%, and 91.9% of the isolates were susceptible to Imipenem, Amikacin and Meropenem respectively. Frequently detected resistance genes were blaTEM-83.8% (134/160), and, blaCTX-M 83.1% (133/160) followed by blaSHVgenes 66.3% (106/160). Co-existence of blaCTX-M, blaTEM and blaSHV was seen in 94/160 (58.8%), blaCTX-M and blaTEM in 118/160 (73.8%), blaTEM and blaSHV in 97/160 (60.6%) and blaCTX-M and blaSHV in 100/160 (62.5%) of isolates tested. Our results indicate a high prevalence of bacteremia from ESBL Enterobacteriaceae in this population of children. These are resistant to commonly used antibiotics and careful choice of antibiotic treatment options is critical. Further studies to evaluate transmission dynamics of resistance genes could help in the reduction of ESBL resistance in these settings.