Comparison of three serological chemiluminescence immunoassays for SARS-CoV-2, and clinical significance of antibody index with disease severity

The clinical significance of the quantitative value of antibodies in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains mostly unidentified. We investigated the dynamics and clinical implications of the SARS-CoV-2 antibody over time using three automated chemiluminescence...

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Bibliographic Details
Published in:PloS one 2021-06, Vol.16 (6), p.e0253889-e0253889
Main Authors: Lee, Nuri, Jeong, Seri, Park, Min-Jeong, Song, Wonkeun
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Automation
Biology and life sciences
Chemiluminescence
Clinical significance
Coronaviruses
Correlation
Correlation coefficient
Correlation coefficients
COVID-19
Disease transmission
Evaluation
Hospitals
IgG antibody
Immunoassay
Immunoassays
Immunoglobulin G
Immunoglobulin M
Infections
Laboratories
Medical schools
Medical tests
Medicine
Medicine and health sciences
Methods
Mortality
Nucleocapsids
Patients
Physical Sciences
Reagents
Research and Analysis Methods
Respiratory diseases
Serology
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Syphilis
Viral diseases
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Summary:The clinical significance of the quantitative value of antibodies in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains mostly unidentified. We investigated the dynamics and clinical implications of the SARS-CoV-2 antibody over time using three automated chemiluminescence immunoassays targeting either nucleocapsids or spikes. A total of 126 specimens were collected from 23 patients with confirmed and indeterminate COVID-19 identified by molecular tests. SARS-CoV-2 antibody index was measured using SARS-CoV2 IgG reagent from Alinity (Abbott) and Access (Beckman Coulter) and SARS-CoV2 Total (IgG + IgM) from Atellica (Siemens). Three immunoassays showed strong correlations with each other (range of Pearson' s correlation coefficient (r) = 0.700-0.854, P < 0.001). Eleven (8.7%) specimens showed inconsistencies. SARS-CoV-2 IgG showed a statistically significantly higher value in patients with severe disease than that in non-severe disease patients (P < 0.001) and was significantly associated with clinical markers of disease severity. The quantitative value of the SARS-CoV-2 IgG antibody measured using automated immunoassays is a significant indicator of clinical severity in patients with COVID-19.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0253889