Evaluation of a point-of-care diagnostic to identify glucose-6-phosphate dehydrogenase deficiency in Brazil

Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common enzyme deficiency, prevalent in many malaria-endemic countries. G6PD-deficient individuals are susceptible to hemolysis during oxidative stress, which can occur from exposure to certain medications, including 8-aminoquinoline...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2021-08, Vol.15 (8), p.e0009649-e0009649
Main Authors: Zobrist, Stephanie, Brito, Marcelo, Garbin, Eduardo, Monteiro, Wuelton M, Clementino Freitas, Suellen, Macedo, Marcela, Soares Moura, Aline, Advani, Nicole, Kahn, Maria, Pal, Sampa, Gerth-Guyette, Emily, Bansil, Pooja, Domingo, Gonzalo J, Pereira, Dhelio, Lacerda, Marcus VG
Format: Article
Language:English
Subjects:
Aminoquinolines
Biology and Life Sciences
Biosensors
Blood
Confidence intervals
Consent
Dehydrogenase
Dehydrogenases
Diagnosis
Diagnosis, Laboratory
Enzymes
Ethics
Fluorescence
Glucose
Glucose 6 phosphate dehydrogenase
Glucose-6-phosphate dehydrogenase deficiency
Glucosephosphate dehydrogenase
Haemolysis
Hemoglobin
Human diseases
Laboratories
Malaria
Medical tests
Medicine and Health Sciences
Methods
Microscopy
Operating temperature
Oxidative stress
Patients
People and places
Performance evaluation
Phosphates
Point of care testing
Population
Public health
Samples
Sensitivity
Sensitivity analysis
Specificity
Spectrophotometry
Tropical diseases
Vector-borne diseases
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Summary:Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common enzyme deficiency, prevalent in many malaria-endemic countries. G6PD-deficient individuals are susceptible to hemolysis during oxidative stress, which can occur from exposure to certain medications, including 8-aminoquinolines used to treat Plasmodium vivax malaria. Accordingly, access to point-of-care (POC) G6PD testing in Brazil is critical for safe treatment of P. vivax malaria. Methodology/Principal findings This study evaluated the performance of the semi-quantitative, POC STANDARD G6PD Test (SD Biosensor, Republic of Korea). Participants were recruited at clinics and through an enriched sample in Manaus and Porto Velho, Brazil. G6PD and hemoglobin measurements were obtained from capillary samples at the POC using the STANDARD and HemoCue 201+ (HemoCue AB, Sweden) tests. A thick blood slide was prepared for malaria microscopy. At the laboratories, the STANDARD and HemoCue tests were repeated on venous samples and a quantitative spectrophotometric G6PD reference assay was performed (Pointe Scientific, Canton, MI). G6PD was also assessed by fluorescent spot test. In Manaus, a complete blood count was performed. Samples were analyzed from 1,736 participants. In comparison to spectrophotometry, the STANDARD G6PD Test performed equivalently in determining G6PD status in venous and capillary specimens under varied operating temperatures. Using the manufacturer-recommended reference value thresholds, the test's sensitivity at the
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0009649