The X-linked splicing regulator MBNL3 has been co-opted to restrict placental growth in eutherians

• Published in: PLoS biology 2022-04, Vol.20 (4), p.e3001615-e3001615
• Main Authors: Spruce, Thomas, Plass, Mireya, Gohr, André, Ray, Debashish, Martínez de Lagrán, María, Rot, Gregor, Nóvoa, Ana, Burguera, Demian, Permanyer, Jon, Miret, Marta, Zheng, Hong, Swanson, Maurice S, Morris, Quaid, Mallo, Moises, Dierssen, Mara, Hughes, Timothy R, Pernaute, Barbara, Irimia, Manuel
• Format: Article
• Language: English
• Subjects: Alternative splicing; Alternative Splicing - genetics; Amino acid sequence; Animals; Binding; Biological evolution; Biology and Life Sciences; Constraining; Developmental biology; Discovery Report; Embryos; Eutheria - genetics; Eutherians; Evolution; Female; Fetuses; Gene expression; Genetic aspects; Localization; Medicine and Health Sciences; Metabolism; Myc protein; Nucleotide sequence; Placenta; Placenta - metabolism; Polyadenylation; Pregnancy; Proteins; Research and analysis methods; Resource allocation; Ribonucleic acid; RNA; RNA - metabolism; RNA-binding protein; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Splicing; Stem cells; Vertebrates; X chromosomes
• ISSN: 1545-7885; 1544-9173; 1545-7885
• DOI: 10.1371/journal.pbio.3001615

Understanding the regulatory interactions that control gene expression during the development of novel tissues is a key goal of evolutionary developmental biology. Here, we show that Mbnl3 has undergone a striking process of evolutionary specialization in eutherian mammals resulting in the emergence of a novel placental function for the gene. Mbnl3 belongs to a family of RNA-binding proteins whose members regulate multiple aspects of RNA metabolism. We find that, in eutherians, while both Mbnl3 and its paralog Mbnl2 are strongly expressed in placenta, Mbnl3 expression has been lost from nonplacental tissues in association with the evolution of a novel promoter. Moreover, Mbnl3 has undergone accelerated protein sequence evolution leading to changes in its RNA-binding specificities and cellular localization. While Mbnl2 and Mbnl3 share partially redundant roles in regulating alternative splicing, polyadenylation site usage and, in turn, placenta maturation, Mbnl3 has also acquired novel biological functions. Specifically, Mbnl3 knockout (M3KO) alone results in increased placental growth associated with higher Myc expression. Furthermore, Mbnl3 loss increases fetal resource allocation during limiting conditions, suggesting that location of Mbnl3 on the X chromosome has led to its role in limiting placental growth, favoring the maternal side of the parental genetic conflict.