Findaureus: An open-source application for locating Staphylococcus aureus in fluorescence-labelled infected bone tissue slices

Staphylococcus aureus (S. aureus) is a facultative pathogenic bacterium that can cause infections in various tissue types in humans. Fluorescence imaging techniques have been employed to visualize the bacteria in ex-vivo samples mostly in research, aiding in the understanding of the etiology of the...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2024-01, Vol.19 (1), p.e0296854-e0296854
Main Authors: Mandal, Shibarjun, Tannert, Astrid, Löffler, Bettina, Neugebauer, Ute, Silva, Luís Bastião
Format: Article
Language:English
Subjects:
Algorithms
Animals
Arrays
Artificial intelligence
Bacteria
Biology and Life Sciences
Bone and Bones - diagnostic imaging
Bones
Care and treatment
Computer and Information Sciences
Datasets
Diagnosis
Engineering and Technology
Evaluation
Fluorescence
Humans
Image analysis
Image processing
Imaging techniques
Information processing
Localization
Medical imaging
Medical research
Medicine and Health Sciences
Metadata
Mice
Microscopes
Microscopy
Microscopy, Fluorescence
Pelvis
Public software
Research and Analysis Methods
Science Policy
Software
Staphylococcal Infections - diagnostic imaging
Staphylococcal Infections - microbiology
Staphylococcus aureus
Staphylococcus aureus infections
Visualization
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Staphylococcus aureus (S. aureus) is a facultative pathogenic bacterium that can cause infections in various tissue types in humans. Fluorescence imaging techniques have been employed to visualize the bacteria in ex-vivo samples mostly in research, aiding in the understanding of the etiology of the pathogen. However, the multispectral images generated from fluorescence microscopes are complex, making it difficult to locate bacteria across image files, especially in consecutive planes with different imaging depths. To address this issue, we present Findaureus, an open-source application specifically designed to locate and extract critical information about bacteria, especially S. aureus. Due to the limited availability of datasets, we tested the application on a dataset comprising fluorescence-labelled infected mouse bone tissue images, achieving an accuracy of 95%. We compared Findaureus with other state-of-the-art image analysis tools and found that it performed better, given its specificity toward bacteria localization. The proposed approach has the potential to aid in medical research of bone infections and can be extended to other tissue and bacteria types in the future.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0296854