Neutralization of ricin toxin on building interior surfaces using liquid decontaminants

• Published in: PloS one 2024-05, Vol.19 (5), p.e0302967-e0302967
• Main Authors: Richter, William R, Weston, Bailey L, Sunderman, Michelle M, Willenberg, Zach, Ratliff, Katherine, Wood, Joseph P
• Format: Article
• Language: English
• Subjects: Ammonium; Animals; Beans; Bioassays; Biocompatibility; Biological activity; Biology and Life Sciences; Bleaches; Building materials; Buildings; Construction Materials; Contamination; Control; Cytotoxicity; Decontamination; Decontamination (from gases, chemicals, etc.); Decontamination - methods; Discount coupons; Disinfectants - chemistry; Disinfectants - pharmacology; Drywall; Effectiveness; Food contamination & poisoning; Hydrogen peroxide; Hydrogen Peroxide - chemistry; Interior walls; Laboratories; Lectins; Medicine and Health Sciences; Methods; Neutralization; Peracetic acid; Peracetic Acid - chemistry; Peracetic Acid - pharmacology; Peroxides; Physical Sciences; Porous materials; Protein biosynthesis; Protein synthesis; Proteins; Research and Analysis Methods; Ricin; Ricinus communis; Sodium; Sodium hypochlorite; Sodium Hypochlorite - chemistry; Sodium Hypochlorite - pharmacology; Sprays; Substrates; Toxicology; Toxins
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0302967

Ricin is a highly toxic protein, capable of inhibiting protein synthesis within cells, and is produced from the beans of the Ricinus communis (castor bean) plant. Numerous recent incidents involving ricin have occurred, many in the form of mailed letters resulting in both building and mail sorting facility contamination. The goal of this study was to assess the decontamination efficacy of several commercial off-the-shelf (COTS) cleaners and decontaminants (solutions of sodium hypochlorite [bleach], quaternary ammonium, sodium percarbonate, peracetic acid, and hydrogen peroxide) against a crude preparation of ricin toxin. The ricin was inoculated onto four common building materials (pine wood, drywall joint tape, countertop laminate, and industrial carpet), and the decontaminants were applied to the test coupons using a handheld sprayer. Decontamination efficacy was quantified using an in-vitro cytotoxicity assay to measure the quantity of bioactive ricin toxin extracted from test coupons as compared to the corresponding positive controls (not sprayed with decontaminant). Results showed that decontamination efficacy varied by decontaminant and substrate material, and that efficacy generally improved as the number of spray applications or contact time increased. The solutions of 0.45% peracetic acid and the 20,000-parts per million (ppm) sodium hypochlorite provided the overall best decontamination efficacy. The 0.45% peracetic acid solution achieved 97.8 to 99.8% reduction with a 30-min contact time.