3,3'-((3,4,5-trifluoropHenyl)methylene)bis(4-hydroxy-2H-chromen-2-one) inhibit lung cancer cell proliferation and migration

• Published in: PloS one 2024-05, Vol.19 (5), p.e0303186-e0303186
• Main Authors: Luo, Wenhui, Chang, Guoxin, Lin, Dingmei, Xie, Hongyi, Sun, Huilong, Li, Zhibin, Mo, Shirong, Wang, Ruixue, Wang, Yan, Zheng, Zhaoguang
• Format: Article
• Language: English
• Subjects: A549 Cells; Animals; Anticancer properties; Antineoplastic Agents - pharmacology; Antineoplastic drugs; Antitumor activity; Apoptosis; Biocompatibility; Biology and Life Sciences; Cancer therapies; Cell adhesion & migration; Cell culture; Cell growth; Cell Line, Tumor; Cell migration; Cell Movement - drug effects; Cell proliferation; Cell Proliferation - drug effects; Cholecystokinin; Coumarins - chemistry; Coumarins - pharmacology; Drug dosages; Ethanol; Fluorine; Gelatinase A; Gelatinase B; Humans; Immunotherapy; In vivo methods and tests; Kidney cancer; Lung cancer; Lung diseases; Lung Neoplasms - drug therapy; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Matrix metalloproteinase; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - metabolism; Matrix metalloproteinases; Medicine and Health Sciences; Metastasis; Methylene; Mice; Natural products; Phosphorylation; Physical Sciences; Public health; Research and Analysis Methods; Software; Tumors; Xenograft Model Antitumor Assays
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0303186

Lung cancer is a major public health challenge and, despite therapeutic improvements, is the first leading cause of cancer worldwide. The current cure rate from advanced cancer treatment is excessively low. Therefore, it is of great importance to identify novel, potent and less toxic anticancer agents for the treatment of lung cancer. The aim of our research is to synthesize a new biscoumarin 3,3'-((3,4,5-trifluorop -phenyl)methylene)bis(4-hydroxy-2H-chromen-2-one) (C35) as an anticancer agent. C35 was simply prepared by 4-hydroxycoumarin and 3,4,5-trifluorobenzaldehyde under ethanol and its structure was analyzed by spectroscopic analyses. The anti-proliferation effect of C35 was detected using CCK-8 assay. Migration abilities were measured by Transwell assay. The expression of correlated proteins was determined by Western blot. The results showed that C35 displayed strong cytostatic effects on lung cancer cell proliferation. In addition, C35 possessed a significant inhibition of migration by reducing the expression of matrix metalloproteinases-2 (MMP-2) and MMP-9 in lung cancer cells. Furthermore, C35 treatment suppressed the phosphorylation of p38 in lung cancer cells. Moreover, in vivo experiments were carried out, in which we treated Lewis tumor-bearing C57 mice via intraperitoneal injection of C35. Results showed that C35 inhibited tumor growth in vivo. In conclusion, our study demonstrated the anticancer activity of C35 via suppression of lung cancer cell proliferation and migration, which is possibly involved with the inhibition of the p38 pathway.