Riboflavin activated by ultraviolet A1 irradiation induces oxidative DNA damage-mediated mutations inhibited by vitamin C

• Published in: Proceedings of the National Academy of Sciences - PNAS 2007-04, Vol.104 (14), p.5953-5958
• Main Authors: Besaratinia, Ahmad, Kim, Sang-in, Bates, Steven E, Pfeifer, Gerd P
• Format: Article
• Language: English
• Subjects: Animals; Ascorbic Acid - pharmacology; Binding sites; Biochemistry; Biological Sciences; Cell Survival - drug effects; Cell Survival - radiation effects; Cells, Cultured; Cellular biology; Cephalopelvic disproportion; DNA; DNA Damage; DNA Footprinting; DNA Mutational Analysis; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Embryo, Mammalian - cytology; English language learners; Enzymes; Fibroblasts; Fibroblasts - drug effects; Fibroblasts - radiation effects; Genetic mutation; Genomics; Hypothesis testing; Irradiation; Lesions; Mice; Mice, Transgenic; Mutation; Mutation - drug effects; Mutation - radiation effects; Oxidation-Reduction; Proteins; Riboflavin - pharmacology; Transgenes; Ultraviolet radiation; Ultraviolet Rays; Vitamin B; Vitamin C
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0610534104

An increasingly popular theory ascribes UVA (>320-400 nm) carcinogenicity to the ability of this wavelength to trigger intracellular photosensitization reactions, thereby giving rise to promutagenic oxidative DNA damage. We have tested this theory both at the genomic and nucleotide resolution level in mouse embryonic fibroblasts carrying the lambda phage cII transgene. We have also tested the hypothesis that inclusion of a cellular photosensitizer (riboflavin) can intensify UVA-induced DNA damage and mutagenesis, whereas addition of an antioxidant (vitamin C) can counteract the induced effects. Cleavage assays with formamidopyrimidine DNA glycosylase (Fpg) coupled to alkaline gel electrophoresis and ligation-mediated PCR (LM-PCR) showed that riboflavin treatment (1 μM) combined with UVA1 (340-400 nm) irradiation (7.68 J/cm²) or higher dose UVA1 irradiation alone induced Fpg-sensitive sites (indicative of oxidized and/or ring-opened purines) in the overall genome and in the cII transgene, respectively. Also, the combined treatment with riboflavin and UVA1 irradiation gave rise to single-strand DNA breaks in the genome and in the cII transgene determined by terminal transferase-dependent PCR (TD-PCR). A cotreatment with vitamin C (1 mM) efficiently inhibited the formation of the induced lesions. Mutagenicity analysis showed that riboflavin treatment combined with UVA1 irradiation or high-dose UVA1 irradiation alone significantly increased the relative frequency of cII mutants, both mutation spectra exhibiting significant increases in the relative frequency of G:C [rightward arrow] T:A transversions, the signature mutations of oxidative DNA damage. The induction of cII mutant frequency was effectively reduced consequent to a cotreatment with vitamin C. Our findings support the notion that UVA-induced photosensitization reactions are responsible for oxidative DNA damage leading to mutagenesis.