An Infrequent Point Mutation of the p53 Gene in Human Nasopharyngeal Carcinoma

Point mutations in the p53 gene have been detected in a variety of human cancers; the mutations are clustered in four "hot-spots" located in the coding region of exons 5, 7, and 8, which coincide with the four most highly conserved regions of the gene. We report the finding of a heterozygo...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992-07, Vol.89 (14), p.6516-6520
Main Authors: Sun, Yi, Hegamyer, Glenn, Cheng, Yu-Juen, Hildesheim, Allan, Chen, Jen-Yang, I-How Chen, Cao, Ya, Yao, Kai-Tai, Colburn, Nancy H.
Format: Article
Language:English
Subjects:
Base Sequence
Biochemistry
Biological and medical sciences
Biopsies
carcinoma
Carcinoma - genetics
Cell lines
Codons
DNA
Exons
Gene Expression
Genes, p53
Genetic mutation
Genetics
Heterozygote
Humans
man
Medical research
Medical sciences
Molecular Sequence Data
Mutation
Nasopharyngeal Neoplasms - genetics
Oligonucleotides - chemistry
Otorhinolaryngology. Stomatology
p53 gene
p53 genes
Point mutation
Polymerase Chain Reaction
protein p53
RNA, Neoplasm - genetics
Tumor cell line
tumor suppressor genes
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Point mutations in the p53 gene have been detected in a variety of human cancers; the mutations are clustered in four "hot-spots" located in the coding region of exons 5, 7, and 8, which coincide with the four most highly conserved regions of the gene. We report the finding of a heterozygous G→C mutation at codon 280 (exon 8), position 2, of the p53 gene in a nasopharyngeal carcinoma (NPC) cell line, originating from Guangdong, a province in the People's Republic of China that leads the world in NPC incidence. A survey of nasopharyngeal tissues and NPC biopsies revealed that 1 out of 12 NPC samples from Hunan, another province in the People's Republic of China with high NPC incidence, had the same heterozygous mutation at codon 280 of p53, and none of 10 biopsies from Taiwan showed a mutation within exons 5-8 of the p53 gene. No other alteration of gene structure, including gross rearrangement or loss of heterozygosity or abnormality of gene expression was detected in NPC cell lines or NPC biopsies. We conclude from this study that mutational or other alterations of the p53 gene are not common in nasopharyngeal carcinogenesis and that a codon-280 mutation of p53 may be involved in$
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.14.6516